Comparative and population genomics of buckwheat species reveal key determinants of flavor and fertility  

作　　者：Kaixuan Zhang Yuqi He Xiang Lu Yaliang Shi Hui Zhao Xiaobo Li Jinlong Li Yang Liu Yinan Ouyang Yu Tang Xue Ren Xuemei Zhang Weifei Yang Zhaoxia Sun Chunhua Zhang Muriel Quinet Zlata Luthar Mateja Germ Ivan Kreft Dagmar Janovska Vladimir Meglic Barbara Pipan Milen I.Georgiev Bruno Studer Mark A.Chapman Meiliang Zhou 

机构地区：[1]Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,National Crop Genebank Building,Zhongguancun South Street No.12,Haidian District,Beijing 100081,China [2]College of Agronomy,Sichuan Agricultural University,Chengdu 611130,China [3]Annoroad Gene Technology(Beijing)Co.,Ltd,Beijing 100176,China [4]College of Agriculture,Institute of Agricultural Bioengineering,Shanxi Agricultural University,Taigu 030801,Shanxi,China [5]Shanxi Key Laboratory of Minor Crops Germplasm Innovation and Molecular Breeding,Shanxi Agricultural University,Taiyuan 030031,Shanxi,China [6]Tongliao Institute Agricultural and Animal Husbandry Sciences,Tongliao 028015,Inner Mongolia,China [7]Groupe de Recherche en Physiologie Ve´ge´tale(GRPV),Earth and Life Institute-Agronomy(ELI-A),Universite´Catholique de Louvain,Croix du Sud 4-5,bo^ıte L7.07.13,B-1348,Louvain-la-Neuve,Belgium [8]Biotechnical Faculty,University of Ljubljana,1000 Ljubljana,Slovenia [9]Nutrition Institute,Trzaska 40,1000 Ljubljana,Slovenia [10]Gene Bank,Crop Research Institute,Drnovska´507,Prague 6,Czech Republic [11]Agricultural Institute of Slovenia,Hacquetova ulica,Ljubljana,Slovenia [12]Laboratory of Metabolomics,Institute of Microbiology,Bulgarian Academy of Sciences,Plovdiv,Bulgaria [13]Center of Plant Systems Biology and Biotechnology,Plovdiv,Bulgaria [14]Molecular Plant Breeding,Institute of Agricultural Sciences,ETH Zurich,Universitaetstrasse 2,8092 Zurich,Switzerland [15]Biological Sciences,University of Southampton,Life Sciences Building 85,Highfield Campus,Southampton SO171BJ,UK

出　　处：《Molecular Plant》2023年第9期1427-1444,共18页分子植物（英文版）

基　　金：the National Key R&D Program of China(2022YFE0140800);the European Union Horizon 2020 project ECOBREED(771367);the Youth Innovation Program of Chinese Academy of Agricultural Sciences(No.Y2022QC02);Project of Sanya Yazhou Bay Science and Technology City(SCKJ-JYRC-2022-22);National Natural Science Foundation of China(32161143005,31911530772,32111540258);PlantaSYST(SGA No 739582 under FPA No.664620);the BG05M2OP001-1.003-001-C01 project,financed by the European Regional Development Fund through the“Science and Education for Smart Growth”Operational Programme and Slovenian Research Agency,program P4-0077“Genetics and Modern Technologies of Crops”.

摘　　要：Common buckwheat(Fagopyrum esculentum)is an ancient crop with a world-wide distribution.Due to its excellent nutritional quality and high economic and ecological value,common buckwheat is becoming increasingly important throughout the world.The availability of a high-quality reference genome sequence and population genomic data will accelerate the breeding of common buckwheat,but the high heterozygosity due to the outcrossing nature has greatly hindered the genome assembly.Here we report the assembly of a chromosome-scale high-quality reference genome of F.esculentum var.homotropicum,a homozygous self-pollinating variant of common buckwheat.Comparative genomics revealed that two cultivated buckwheat species,common buckwheat(F.esculentum)and Tartary buckwheat(F.tataricum),underwent metabolomic divergence and ecotype differentiation.The expansion of several gene families in common buckwheat,including FhFAR genes,is associated with its wider distribution than Tartary buckwheat.Copy number variation of genes involved in the metabolism of flavonoids is associated with the difference of rutin content between common and Tartary buckwheat.Furthermore,we present a comprehensive atlas of genomic variation based on whole-genome resequencing of 572 accessions of common buckwheat.Population and evolutionary genomics reveal genetic variation associated with environmental adaptability and floral development between Chinese and non-Chinese cultivated groups.Genome-wide association analyses of multi-year agronomic traits with the content of flavonoids revealed that Fh05G014970 is a potential major regulator of flowering period,a key agronomic trait controlling the yield of outcrossing crops,and that Fh06G015130 is a crucial gene underlying flavor-associated flavonoids.Intriguingly,we found that the gene translocation and sequence variation of FhS-ELF3 contribute to the homomorphic self-compatibility of common buckwheat.Collectively,our results elucidate the genetic basis of speciation,ecological adaptation,fertility,and unique fl

关 键 词：BUCKWHEAT genomics natural variation adaptation FLAVONOIDS FAGOPYRUM 

分 类 号：S51[农业科学—作物学]