海鲜产品中空肠弯曲菌2种检测标准的比较  

Comparison of two standards for detection of two types of Campylobacter jejuni in seafood

作　　者：刘楚云[1] 马艳萍 段永翔[1] 张茂俊[2] 何娇明 鞠长燕[1] LIU Chuyun;MA Yanping;DUAN Yongxiang;ZHANG Maojun;HE Jiaoming;JU Changyan(Microbiological Testing Department,Shenzhen Nanshan Center for Disease Control and Prevention,Shenzhen Guangdong,518054,China;Diagnosis Room,Institute for Infectious Disease Prevention and Control,Chinese Center for Disease Control and Prevention,Beijing,102206,China)

机构地区：[1]深圳市南山区疾病预防控制中心微生物检验科,广东深圳518054 [2]中国疾病预防控制中心传染病预防控制所诊断室,北京102206

出　　处：《职业与健康》2023年第18期2494-2498,共5页Occupation and Health

基　　金：深圳市基金项目(No.SZSM201803081);深圳市南山区基金项目(南科研卫第2020049号);深圳市南山区基金项目(传染病防控重点学科)。

摘　　要：目的 利用GB 4789.9—2014《食品安全国家标准食品微生物学检验空肠弯曲菌检验》(以下简称GB4789.9法)与T/CPMA 006—2019《空肠弯曲菌、结肠弯曲菌检验方法》(以下简称T/CPMA 006法)2种标准方法检测贝类样品中空肠弯曲菌的阳性率,并用模拟样本以2种检测标准对空肠弯曲菌的最低检出浓度及检出率差别开展讨论。方法 2020年11—12月采集深圳市某市场39份贝类海鲜样品,分别用GB4789.9和T/CPMA 006的2种标准方法进行空肠弯曲菌检测,对于检测阴性的标本,实验室掺入终浓度分别为100、101、102、103、104、105CFU/g的活菌进行模拟实验,每个浓度模拟3个样品,分析比较2种标准方法的最低检出浓度。根据最低检出浓度,实验室掺入终浓度分别为103和104CFU/g的空肠弯曲菌活菌,每个浓度各10份样品,比较2种标准方法的检出率差异。利用基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser desorption ionization-time of flight mass spectrometry,MALDI-TOF)和荧光聚合酶链反应(polymerase chain reaction,PCR)作为菌落验证和辅助检测。结果 39份原始样品中T/CPMA 006检测标准的空肠弯曲菌阳性率为2.6%(1/39),而GB 4789.9标准方法均未检出。模拟样本的结果显示,GB 4789.9和T/CPMA 006的最低检出浓度分别是103和101CFU/g,T/CPMA 006比GB 4789.9的最低检出浓度低2个数量级。当样品中空肠弯曲菌终浓度为103和104CFU/g时,GB 4789.9的检出率分别为10%(1/10)和20%(2/10),过滤培养法的检出率皆为100%(10/10)。结论 海鲜中也存在空肠弯曲菌的污染,此前国内未见报道。与GB 4789.9标准方法相比,T/CPMA 006标准可显著提高海鲜样品中空肠弯曲菌的检出率,并将最低检出浓度降低2个数量级。Objective To detect the positive rate of Campylobacter jejuni in shellfish samples by GB 4789.9-2014 National Food Safety Standard Food Microbiological Examination Examination of Campylobacter jejuni(hereinafter referred to GB 4789.9 method)and T/CPMA 006-2019 Examination of Campylobacter jejuni and Campylobacter coli(hereinafter referred to T/CPMA 006 method),discuss the differences in the minimum detection concentration and detection rate of Campylobacter jejuni between two methods with artificially contaminated samples.Methods From November to December 2020,39 shellfish samples were collected from a market in Shenzhen City,and the Campylobacter jejuni was detected by GB 4789.9 method and T/CPMA 006 method.The negative samples were artificially contaminated with 100,101,102,103,104 and 105 CFU/g Campylobacter jejunifor the simulation experiment,three samples for each concentration,to analyze and compare the minimum detection concentration of two standard methods.According to the minimum detection concentration,the samples were contaminated with 103 and 104 CFU/g Campylobacter jejuni,10 samples for each concentration,to compare the detection rate of two standard methods.The matrixassisted laser desorption ionization-time of flight mass spectrometry(MALDI-TOF)and fluorescent polymerase chain reaction(PCR)were used for the colony validation and auxiliary testing.Results Among 39 original samples,the positive rate of Campylobacter jejuniby T/CPMA 006 method was 2.6%(1/39),and on sample was detected by GB4789.9 method.Among the artificially contaminated samples,the minimum detection concentration by GB 4789.9 method and T/CPMA 006 method was 103 and 101CFU/g,and the minimum detection concentration of T/CPMA 006 was 2 orders of magnitude lower than that of GB 4789.9. Whenthe final concentration of Campylobacter jejuni was 103 and 101 CFU/g,the detection rate by GB 4789.9 was 10%(1/10) and 20%(2/10) respectively,while the detection rate of filtration culture method were both 100%(10/10). Conclusions The Campylobacterj

关 键 词：空肠弯曲菌 海鲜 基质辅助激光解吸电离飞行时间质谱 荧光聚合酶链反应 

分 类 号：TS254.7[轻工技术与工程—水产品加工及贮藏工程]