A highly selective fluorescent probe for visualizing dry eye disease-associated viscosity variations  

作　　者：Lili Lian Ruirui Zhang Shuai Guo Zhenmin Le Lixiong Dai Yueping Ren Xiao-Qi Yu Ji-Ting Hou Jianliang Shen 

机构地区：[1]National Engineering Research Center of Ophthalmology and Optometry,Eye Hospital,Wenzhou Medical University,Wenzhou 325027,China [2]Key Laboratory of Green Chemistry and Technology(Ministry of Education),College of Chemistry,Sichuan University,Chengdu 610064,China [3]National Clinical Research Center for Ocular Diseases,Eye Hospital,Wenzhou Medical University,Wenzhou 325027,China [4]Wenzhou Institute,University of Chinese Academy of Sciences,Wenzhou 325000,China

出　　处：《Chinese Chemical Letters》2023年第10期168-173,共6页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.22075281);Zhejiang Provincial Natural Science of Foundation of China(No.LZ21B010001);University of Chinese Academy of Science(No.WIUCASQD2020008);。

摘　　要：Dry eye disease(DED) is a multifactorial chronic inflammatory disease of the ocular surface with complex and unclear etiology. The development of reliable detection tools for the pathology of DED will benefit its treatment, but it is still lacking. In parallel, it has been discovered recently that viscosity changes are involved in inflammation processes. In this regard, we constructed a fluorescent probe V5with an asymmetric donor-acceptor-donor(D-A-D) feature after rational structural modulation for viscosity detection during DED progression. The probe manifested a remarkable fluorescence enhancement(110 folds) in highly viscous conditions without interferences from polarity and reactive species. Specifically, no aggregation effect of the probe was found in glycerol. Moreover, viscosity increment in human corneal epithelial cells(HCECs) induced by hyperosmosis and inflammation was monitored, and ferroptosis in HCECs also led to the viscosity elevation. A reactive oxygen species(ROS)-dependent viscosity changes during DED progression is demonstrated. Finally, viscosity change in corneal epithelial cell layer from mice treated by scopolamine was also visualized for the first time. We anticipate this work can provide a new lens to the pathogenesis study and diagnosis of DED and other ophthalmic diseases using fluorescence methods.

关 键 词：Fluorescent probe VISCOSITY BIOIMAGING Corneal tissue Dry eye disease 

分 类 号：R777.34[医药卫生—眼科] O657.3[医药卫生—临床医学]