清肠温中方对溃疡性结肠炎小鼠AhR/IL-22信号通路的影响  

The effect of Qingchang Wenzhong Decoction on the AhR/IL-22 signaling pathway in mice with ulcerative colitis

作　　者：王木源 李军祥[1] 毛堂友 袁亚利 孙中美 史瑞[1] 王志斌[1] 石磊[1] 张文基 王佳丽 陈晓伟[1] WANG Muyuan;LI Junxiang;MAO Tangyou;YUAN Yali;SUN Zhongmei;SHI Rui;WANG Zhibin;SHI Lei;ZHANG Wenji;WANG Jiali;CHEN Xiaowei(Department of Gastroenterology,Dongfang Hospital,Beijing University of Chinese Medicine,Beijing,100078,China;Center for Experimental Medicine,Dongfang Hospital,Beijing University of Chinese Medicine;Department of Gastroenterology,Tianjin Nankai Hospital)

机构地区：[1]北京中医药大学东方医院脾胃肝胆科,北京100078 [2]北京中医药大学东方医院实验医学中心 [3]天津市南开医院消化内科

出　　处：《中国中西医结合消化杂志》2023年第10期788-792,797,共6页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

基　　金：首都卫生发展科研专项项目(No:首发2022-4-4205);国家自然科学青年基金(No:81903990);中华中医药学会(2022—2024年度)青年人才托举工程项目(No:CACM-2022-QNRC2-A02)。

摘　　要：目的:研究清肠温中方对葡聚糖硫酸钠(DSS)诱导的溃疡性结肠炎(UC)小鼠AhR/IL-22信号通路的影响。方法:将28只SPF级健康雌性C57BL/6小鼠随机分为空白组、模型组、清肠温中方低剂量组、清肠温中方中剂量组、清肠温中方高剂量组、美沙拉秦组。空白组小鼠全程自由进食饮水,模型组和清肠温中方低、中、高各剂量组及美沙拉秦组均采用自由饮用2.5%(w/v)DSS溶液7 d复制UC模型,第8天开始模型组给予去离子水灌胃,清肠温中方各剂量组给予相应浓度的中药干预1周,美沙拉秦组给予美沙拉秦水溶液干预1周。期间每日观察小鼠一般情况,测量体重、检测便潜血、记录粪便性状,计算疾病活动指数(DAI)。干预结束后留取结肠组织,Western Blot检测AhR、IL-22蛋白水平,RT-qPCR测定AhR、IL-22mRNA的相对表达量。结果:与空白组比较,UC小鼠呈现明显的肠道炎症,表现出不同程度的便血、腹泻、体重下降等,清肠温中方中剂量组和美沙拉秦组小鼠体重较模型组明显升高(P<0.05);清肠温中方中剂量组小鼠较模型组DAI下降明显(P<0.05)。清肠温中方组AhR、IL-22蛋白表达量较模型组升高明显(P<0.05);清肠温中方组AhR、IL-22mRNA表达量较模型组显著升高(P<0.01)。结论:清肠温中方可能通过激活UC小鼠中AhR/IL-22信号通路,达到治疗UC的目的。Objective To study the effect of Qingchang Wenzhong Decoction(QCWZD)on dextran sodium sulfate(DSS)-induced AhR/IL-22 signaling pathway in mice with ulcerative colitis(UC).Methods Twenty-eight healthy female C57BL/6 mice were randomly divided into control group,DSS group,QCWZD low-dose group,QCWZD medium-dose group,QCWZD high-dose group,Mesalazine group.Mice in control group were fed and watered freely throughout the whole process.DSS group,doses group of QCWZD and Mesalazine group were used to replicate the UC model by freely drinking 2.5%(w/v)DSS solution for 7 days.On day 8,the model group was given deionized water for gavage,and each dose of the QCWZD was given the corresponding concentration of Chinese medicine for 1 week in DSS group,and the Mesalazine solution for 1 week in the Mesalazine group.During this period,the mice were observed daily for general condition,weight measurement,detection of fecal occult blood,recording of fecal properties,and calculation of disease activity index(DAI).At the end of the intervention,colonic tissues were collected and the levels of AhR and IL-22 protein were measured,and the levels of AhR/IL-22mRNA were determined.Results Compared with control group,UC mice showed obvious intestinal inflammation,exhibiting different degrees of blood in stool,diarrhea and weight loss,etc.The body weight of mice in QCWZD medium-dose group and Mesalazine group was significantly higher than that in DSS group(P<0.05);the DAI of mice in the medium-dose group of QCWZD decreased significantly compared with that in DSS group(P<0.05).The expressions of AhR and IL-22 protein in QCWZD group were significantly higher than those in DSS group(P<0.05);the expressions of AhR/IL-22mRNA in QCWZD group were significantly higher than those in DSS group(P<0.01).Conclusion QCWZD maybe used to treat UC by activating the AhR/IL-22 signaling pathway in UC mice.

关 键 词：溃疡性结肠炎 清肠温中方 AhR/IL-22信号通路 肠黏膜修复 

分 类 号：R574.62[医药卫生—消化系统]