1株药用红树内生真菌Talaromyces flavus TGGP35次级代谢产物及其抗氧化活性研究  

Study on the secondary metabolites and their antioxidant activities of a medicinal mangrove endophytic fungus Talaromyces flavus TGGP35

作　　者：蔡瑾 陈敏静 李诗川 刘婧 罗运展 张学龙 李杲钰 肖媚 韦方芳 黄国雷 郑彩娟[1] CAI Jin;CHEN Minjing;LIU Shichuan;LIU Jing;LUO Yunzhan;ZHANG Xuelong;LI Gaoyu;XIAO Mei;WEI Fangfang;HUANG Guolei;ZHENG Caijuan(Key Laboratory of Tropical Medicinal Resource Chemistry of Ministry of Education,College of Chemistry and Chemical Engineering,Hainan Normal University,Haikou 571158,China;Hainan Dongzhaigang National Nature Reserve Administration,Haikou 570101,China)

机构地区：[1]海南师范大学热带药用资源化学教育部重点实验室,化学与化工学院,海南海口571158 [2]海南东寨港国家级自然保护区管理局,海南海口570101

出　　处：《中国海洋药物》2023年第5期59-64,共6页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金项目(32160108);海南省重点研发计划项目(ZDYF2021SHFZ270);海南省自然科学基金项目(220RC 593);海南省重大科技计划项目(ZDKJ202008);海南省院士创新平台科研专项资金(YSPTZX202030)资助。

摘　　要：目的研究1株药用红树老鼠簕(Acanthus ilicifolius L.)来源篮状属真菌Talaromyces flavus TGGP35的次级代谢产物及其抗氧化活性。方法综合利用正相硅胶柱层析、Sephadex LH-20凝胶柱层析、半制备高效液相色谱(HPLC)等色谱分离方法,对TGGP35液体发酵产物的乙酸乙酯粗浸膏进行系统分离纯化;运用核磁共振波谱(1D NMR)、质谱(MS)等方法并结合文献报道数据确定化合物结构,并对其抗氧化活性进行测试。结果从真菌T.flavus TGGP35发酵产物中分离得到9个化合物,包括2个生物碱类化合物thymidine(1)和2’-O-methyluridine(2),3个内酯类化合物aspergilactone A(3)、aspergilactone B(4)和nafuredin(5),1个糖苷类化合物carnemycin E(6),3个二酮哌嗪类化合物cyclo-(L-Val-L-Pro)(7)、cyclo(S)-Pro-8-hydroxy-(R)-Ile(8)和cyclo(L-Pro-L-Leu)(9),其中化合物6表现出一定的抗氧化活性,其半抑制浓度(IC_(50))为0.37 mmol/L。结论化合物1~4和6为首次从篮状属真菌Talaromyces sp.中分离得到,发现1个具有中等抗氧化活性的化合物,具有一定的研究和应用价值。Objective To study the secondary metabolites and antioxidant activities of the fungus Talaromyces flavus TGGP35 derived from medicinal mangrove Acanthus ilicifolius L.Methods The compounds were separated by normal phase silica gel,Sephadex LH-20,semi-preparative HPLC column chromatography,thin layer chromatography and so on.And their structures were identified by 1D NMR,MS,other spectral analysis methods and comparison with literature data.They were also tested for their antioxidant activity.Results Nine known compounds were isolated from the fungus T flavus TGGP35,including two alkaloids thymidine(1)and 2'-O-methyluridine(2),three lactones aspergilactone A(3),aspergilactone B(4),nafuredin(5),one glycoside carnemycin E(6),and three diketopiperazines cyclo-(L-Val-L-Pro)(7)、cyclo(S)-Pro-8-hydroxy-(R)-Ile(8)andcyclo(L-Pro-L-Leu)(9).Among them,compound 6 showed antioxidant inhibitory activity with IC_(50) value of 0.37 mmol/L.Conclusion Compounds 1-4 and 6 were isolated from the genus Talaromyces sp.for the first time,and compound 6 showed moderate antioxidant activity,these results showed important research and application value.

关 键 词：内生真菌 Talaromyces flavus 次级代谢产物 抗氧化活性 

分 类 号：R931.77[医药卫生—生药学]