XIAP与XAF1异常表达与卵巢癌的相关性分析  

Correlation analysis of abnormal expression of XIAP and XAF1 with ovarian cancer

作　　者：刘星辰 刘娟 魏宝宝 刘洁 刘辉 Liu Xingchen;Liu Juan;Wei Baobao;Liu Jie;Liu Hui(Department of Obstetrics and Gynecology,The Sith People's Hospital of Chengdu,Chengdu 610051,Sichuan Province,China;Department of Obstetrics and Gynecology,Key Laboratory of Birth Defects and Related Diseases of Women and Children(Sichuan University),Ministry of Education,West China Second University Hospital,Sichuan University,Chengdu 61004l,Sichuan Province,China)

机构地区：[1]成都市第六人民医院妇产科,成都610051 [2]四川大学华西第二医院妇产科、出生缺陷与相关妇儿疾病教育部重点实验,成都610041

出　　处：《中华妇幼临床医学杂志（电子版）》2023年第4期419-427,共9页Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition)

基　　金：四川省科技厅科技支撑计划项目(2018SZ0164)。

摘　　要：目的:探讨X染色体连锁凋亡抑制蛋白(XIAP)与XIAP相关因子(XAF1)异常表达与卵巢癌的相关性。方法:选择2018年6月至12月于四川大学华西第二医院妇科进行卵巢癌患者全面临床分期手术,经术后切除卵巢癌组织的活组织病理学检查,被诊断为卵巢癌的72例患者为研究对象,纳入卵巢癌组;进一步按照组织病理学检查结果将其分为中-高分化亚组(n=24)与低分化亚组(n=48),浆液性癌亚组(n=36)与非浆液性癌亚组(n=36);按照国际妇产科联盟(FIGO)临床分期进一步分为Ⅰ~Ⅱ期亚组(n=35)与Ⅲ~Ⅳ期亚组(n=37),淋巴结未转移亚组(n=59)与淋巴结转移亚组(n=13)。选择同期在同一家医院,因卵巢畸胎瘤、卵巢囊肿等进行患侧卵巢手术,并经术后切除组织的活组织病理学检查,被诊断为良性卵巢肿瘤的35例良性卵巢肿瘤患者,以及因子宫腺肌病、子宫肌瘤等,进行全子宫及双附件切除术,并经术后切除组织的活组织病理学检查,被诊断为子宫腺肌病、子宫肌瘤的卵巢功能正常的30例患者作为对照,分别纳入良性肿瘤组与对照组。采用免疫组织化学(IHC)法检测3组患者手术切除卵巢组织的XIAP与XAF1表达情况。对3组患者手术切除卵巢组织的XIAP与XAF1阳性表达率比较,采用χ^(2)检验。采用Spearman秩相关性分析,对卵巢癌组患者XAF1与XIAP表达水平相关性进行分析。3组患者年龄等一般临床资料比较,差异无统计学意义(P>0.05)。本研究遵循的程序符合2013年修订的《世界医学协会赫尔辛基宣言》要求。结果:①卵巢癌组、良性肿瘤组及对照组患者卵巢组织的XIAP阳性表达率分别为81.3%(60/72)、51.4%(18/35)、46.7%(14/30),XAF1阳性表达率分别为38.9%(28/72)、80.0%(28/35)、86.7%(26/30),3组患者卵巢组织的XIAP、XAF1阳性表达率分别总体比较,差异均有统计学意义(χ^(2)=18.12、28.06,P<0.001)。进一步对其分别进行两两比较结果显示,卵巢癌组患�Objective To explore the correlation of abnormal expression of X-linked inhibitor of apoptosis protein(XIAP)and XIAP associated factor(XAF)1 with ovarian cancer.Methods A total of 72 patients diagnosed with ovarian cancer based on postoperative histopathological examination following comprehensive staging surgery in the Department of Gynecology,West China Second University Hospital,Sichuan University from June to December 2018 were selected as subjects,and were included into ovarian cancer group.Based on results of histopathological examination,they were further divided into moderately and well-differentiated subgroup(n=24)and poorly differentiated subgroup(n=48),serous carcinoma subgroup(n=36)and non-serous carcinoma subgroup(n=36);according to the International Federation of Gynecology and Obstetrics(FIGO)clinical staging,they were further divided into stage Ⅰ-Ⅱ subgroup(n=35)and stage Ⅲ-Ⅳ subgroup(n=37),without lymph node metastasis subgroup(n=59)and lymph node metastasis subgroup(n=13).And 35 ovarian benign tumor patients who underwent unilateral ovarian cystectomy for ovarian teratoma,ovarian cyst,and so on in the same hospital during the same period and were histopathologically diagnosed with benign ovarian tumors postoperatively,were included into benign tumor group.And 30 patients who underwent total hysterectomy with bilateral salpingo-oophorectomy for adenomyosis and uterine fibroids and were histopathologically diagnosed with adenomyosis and uterine fibroids,while maintainingnormal 1ovarianfunction,,were included into normal control group.Immunohistochemistry(IHC)was used to detect the expression of XIAP and XAF1 in surgically excised ovarian tissues of three groups.Chi-square test were conducted for comparison of positive expression rates of XIAP and XAF1 in surgically excised ovarian tissue among three groups.Spearman's rank correlation analysis was used to analyze the correlation between XAF1 and XIAP expression in patients of ovarian cancer group.There were no statistical differences among

关 键 词：卵巢肿瘤 X连锁凋亡抑制蛋白质 X连锁凋亡抑制蛋白质相关因子 免疫组织化学 肿瘤分期 细胞分化 淋巴结转移 妇女 

分 类 号：R737.31[医药卫生—肿瘤]