基于NF-κB信号通路探讨通管助孕方治疗输卵管阻塞性不孕症大鼠的作用机制  

Explore the mechanism of Tongguan Zhuyun Decoction in the treatment of tubal obstructive infertility rats based on NF-κB signal pathway

作　　者：李盼盼 高慧[2] 刘玉兰[2] 扈有芹[3] 吴斯怡 张晴爽 LI Panpan;GAO Hui;LIU Yulan;HU Youqin;WU Siyi;ZHANG Qingshuang(Chengde Medical University,Chengde 067000,China;Affiliated Hospital of Chengde Medical University,Chengde 067000,China;Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine,Cangzhou 061000,China)

机构地区：[1]承德医学院,承德067000 [2]承德医学院附属医院,承德067000 [3]沧州中西医结合医院,沧州061000

出　　处：《天津中医药大学学报》2023年第5期600-608,共9页Journal of Tianjin University of Traditional Chinese Medicine

基　　金：国家中医药管理局高慧全国名老中医药专家传承工作室建设项目(2015048157);河北省中医药重点学科(中医妇科学)建设项目(202200505334);河北省中医药管理局中医药类科研计划课题项目(2019199,2018164,2017082)。

摘　　要：[目的]基于核转录因子-κB(NF-κB)信号通路探讨通管助孕方治疗输卵管阻塞性不孕症的作用机制。[方法]将140只雌性SD大鼠随机分为正常组、模型组、中成药组、西药组及通管助孕方高、中、低剂量组,每组20只。除正常组外,其余各组采用金黄色葡萄球菌建立输卵管阻塞性不孕症大鼠模型,造模后第7天,各组分别给予生理盐水、丹黄祛瘀胶囊混悬液、人胎盘组织液、通管助孕方高、中、低剂量治疗21 d后,药效组大鼠取材处理,生殖组大鼠进行雌雄合笼实验。采用酶联免疫吸附(ELISA)法检测各组大鼠血清中NF-κB、核因子κB抑制蛋白(IκB)、环氧合酶2(COX-2)、前列腺素E2(PGE2)的水平,采用蛋白免疫印迹(Western Blot)法和实时定量荧光聚合酶链反应(RT-PCR)法分别检测输卵管组织中NF-κB、IκB、COX-2、PGE2的蛋白和基因表达情况。采用肉眼观察以及光镜下观察输卵管组织病理形态学变化。观察并记录生殖组大鼠受孕及产仔情况。[结果] 1)与正常组比较,模型组大鼠NF-κB、COX-2、PGE2蛋白和基因表达升高,IκB蛋白和基因表达降低(P<0.05);与模型组比较,通管助孕方高、中、低剂量组NF-κB、COX-2、PGE2蛋白和基因表达降低,IκB蛋白和基因表达升高(P<0.05);与中成药组及西药组比较,通管助孕方高剂量组中NF-κB、COX-2、PGE2蛋白和基因表达降低,IκB蛋白和基因表达升高(P<0.05)。2)通过肉眼观察及光镜观察后,与模型组比较,各给药组输卵管组织形态均有不同程度改善,尤以通管助孕方高剂量组改善最为明显,基本接近正常。3)对生殖功能的影响:模型组妊娠率为0%,正常组妊娠率为100%,两组比较差异有统计学意义(P<0.05),表明金黄色葡萄球菌接种法可以成功建立输卵管阻塞性不孕症大鼠模型。通管助孕方高、中、低剂量组妊娠率及平均产仔数与模型组比较,差异有统计学意义(P<0.05),说明通管助孕方�[Objective]To explore the mechanism of Tongguan Zhuyun Decoction in the treatment of tubal obstructive infertility rats based on NF-κB signal pathway.[Methods]The 140 female SD rats were randomly divided into normal group,model group,Chinese patent medicine group,Western medicine group and Tongguan Zhuyun Decoction high,medium and low dose groups,with 20 rats in each group.Except the normal group,the other groups were injected with staphylococcus aureus to establish the rat model of tubal obstructive infertility.On the 7th day after modeling,each group was given physiological salt water,Danhuang Quyu Decoction suspension,human placental tissue fluid,Tongguan Zhuyun Decoction high,medium and low dose were treated for 21 d,the rats in the pharmacodynamic group were sampled and treated,and the rats in the reproductive group were used for the male female cage experiment.ELISA was used to detect the levels of NF-κB,IκB,COX-2 and PGE2 in serum of rats in each group.The protein and gene expression levels of NF-κB,IκB,COX-2 and PGE2 were detected by Western blot and RT-PCR.The pathomorphological changes of fallopian tube were observed by naked eye and light microscope.Observe and record the pregnancy and delivery of rats in the reproductive group.[Results]1)Compared with the normal group,the expression levels of NF-κB,COX-2,PGE2 protein and gene in the fallopian tube of the model group increased significantly,the expression levels of IκB protein and gene decreased significantly(P<0.05);compared with the model group,the expression levels of NF-κB,COX-2,PGE2 protein and gene in rat fallopian tube tissue of Tongguan Zhuyun Decoction high,medium and low dose groups decreased significantly,the expression levels of IκB protein and gene increased significantly(P<0.05);compared with Chinese patent medicine group and Western medicine group,the expression levels of NF-κB,COX-2,PGE2 protein and gene in rat fallopian tube tissue of Tongguan Zhuyun Decoction high-dose group decreased significantly,the expression levels of I

关 键 词：通管助孕方 输卵管阻塞性不孕症 NF-ΚB IΚB COX-2 PGE2 

分 类 号：R285.5[医药卫生—中药学]