微囊藻毒素暴露对河川沙塘鳢病理损伤、生化反应的影响  

Effects of Microcystin-LR Exposure on Pathological Damage and Biochemical Reaction of Odontobutis Potamophila

作　　者：郑友[1,2] 姜虎成 张燕[1,2] 李杰 周梓涵 刘国兴 ZHENG You;JIANG Hucheng;ZHANG Yan;LI Jie;ZHOU Zihan;LIU Guoxing(Freshwater Fisheries Research Institute of Jiangsu Province,Nanjing,Jiangsu 210017,China;The Low-temperature Germplasm Bank of Important Economic Fish of Jiangsu Provincial Science and Technology Resources(Agricultural Germplasm Resources)Coordination Service Platform,Nanjing,Jiangsu 210017,China;College of Animal Science and Technology,Yangzhou University,Yangzhou,Jiangsu 225009,China)

机构地区：[1]江苏省淡水水产研究所,江苏南京210017 [2]江苏省科技资源(农业种质)统筹服务平台重要经济鱼类低温种质库,江苏南京210017 [3]扬州大学动物科学与技术学院,江苏扬州225009

出　　处：《水产养殖》2023年第11期22-27,共6页Journal of Aquaculture

基　　金：江苏省农业重大新品种创制项目(PZCZ201743);江苏现代农业产业技术体系建设专项资金(JATS[2022]415,JATS[2022]417);水产良种保种(沙塘鳢)(2022-SJ-012)。

摘　　要：开展了微囊藻毒素暴露对河川沙塘鳢病理损伤、生化反应的影响试验。设置3个试验组(D3、D5、D7)1个对照组(CK),每组设3个重复组,于试验的第0、3、5、7天,随机取每组3尾河川沙塘鳢的肠道和肝脏组织,进行生化分析和组织病理学观察。结果表明,微囊藻毒素会导致肠道黏膜肌层空泡化,同时引起隐窝结构异常,影响肠道营养物质吸收。肝脏同时出现严重损伤,主要包括肝细胞溶解和充血等症状。随着暴露时间的增加,肠道总超氧化物歧化酶活性受到抑制,过氧化氢酶活性和丙二醛的浓度变化,呈现先下降后上升的趋势,谷胱甘肽过氧化物酶和还原型谷胱甘肽的浓度呈现相反的趋势。这些结果表明了微囊藻毒素对河川沙塘鳢的肠道的损伤可能是通过产生过量活性氧引起的。The effects of Microcystin-LR(MC-LR)exposure on pathological damage and biochemical reaction of Odontobutis potamophila were studied.Three experimental groups(D3,D5,and D7)and one control group(CK)were set up,and there were three repetitive groups in each group.On the 0th,3rd,5th,and 7th days of the experiment,the intestinal and liver tissues of 3 Odontobutis potamophila were randomly selected for biochemical analysis and histopathological observation.The results showed that MC-LR could lead to the vacuolization of the muscular layer of intestinal mucosa and the abnormal structure of crypt,which affected the absorption of intestinal nutrients.Severe liver injury occurred at the same time,including hepatolysis and hyperemia.With the increase of exposure time,the activity of total superoxide dismutase in intestinal tract was inhibited,and the concentration of catalase and malondialdehyde decreased at first and then increased.The concentration of glutathione peroxidase and reduced glutathione showed the opposite trend.These findings imply that the increased generation of reactive oxygen species may contribute to the intestinal damage brought on by MC-LR.

关 键 词：沙塘鳢 藻毒素 组织损伤 酶活 肠道 

分 类 号：X174[环境科学与工程—环境科学]