维生素D受体基因与NF-κB通路在急性淋巴细胞白血病患儿血液中的表达及意义  

Expression and Significance of Vitamin D Receptor Gene and NF-κB Pathway in Blood of Children with Acute Lymphoblastic Leukemia

作　　者：伊丽苏娅·帕尔哈提 海力其古丽·努日丁[1] 严媚[1] Yiisuya Paerhati;Haiiqigui Nuriding;YAN Mei(Department of Pediatrics,The First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,Xinjiang Uygur Autonomous Region,China)

机构地区：[1]新疆医科大学第一附属医院儿科中心,新疆乌鲁木齐830054

出　　处：《中国实验血液学杂志》2023年第6期1624-1628,共5页Journal of Experimental Hematology

基　　金：新疆维吾尔自治区天山创新团队计划项目(2020D14027);新疆维吾尔自治区自然科学基金面上项目(2021D01C323)。

摘　　要：目的:探讨维生素D受体(VDR)基因与NF-κB通路在儿童急性淋巴细胞白血病(ALL)中的表达情况、临床意义及对预后的影响。方法:选取2018年12月至2021年12月明确诊断为ALL的30例患儿作为病例组,30名健康体检儿童作为对照组;采集所有研究对象外周血,通过实时荧光定量PCR检测VDR、NF-κB mRNA表达,Western blot检测VDR、NF-κB蛋白表达,并回顾性分析上述基因mRNA表达情况与患者临床特征间的关系。结果:ALL患儿外周血中VDR mRNA相对表达量明显低于对照组(P<0.05),而NF-κB mRNA相对表达量显著高于对照组(P<0.001)。初诊时外周血白细胞数<50×10^(9)/L的患儿NF-κB mRNA的表达明显高于≥50×10^(9)/L的患儿(P<0.01);感染患儿NF-κB mRNA的表达水平明显高于非感染患儿(P<0.05);不同性别、年龄、初诊时血红蛋白量、血小板数、免疫学分型、危险度与诱导缓解情况的患儿NF-κB mRNA的表达水平比较差异均无统计学意义(P>0.05)。结论:NF-κB表达情况对儿童ALL诊断及预后判断具有较高的价值。Objective:To investigate the expression,clinical significance and prognosis of vitamin D receptor(VDR)gene and NF-κB pathway in children with acute lymphoblastic leukemia(ALL).Methods:Thirty children with definitive diagnoses of ALL from December 2018 to December 2021 were selected as ALL group,and 30 healthy children under physical examination were selected as control group.Peripheral blood of all study subjects was collected.The VDR and NF-κB mRNA and protein expressions were detected by real-time quantitative PCR and Western blot,respectively.The relationship between mRNA expression of the above genes and clinical characteristics of children was retrospectively analyzed.Results:The relative expression of VDR mRNA in peripheral blood of children with ALL was significantly lower than that in the control group(P<0.05),while NF-κB mRNA was higher(P<0.001).The expression of NF-κB mRNA in ALL children with peripheral blood white blood cell count(WBC)<50×10^(9)/L at initial diagnosis was significantly higher than those with WBC≥50×10^(9)/L(P<0.01).The expression of NF-κB mRNA in ALL children with infection was significantly higher than that those without infection(P<0.05).There were no significant differences in NF-κB mRNA expression between children with different sex,age,hemoglobin at initial diagnosis,platelet,immunologic typing,risk and induced response(P>0.05).Conclusion:The expression of NF-κB is of value to diagnosis and prognosis of ALL in children.

关 键 词：急性淋巴细胞白血病 维生素D受体 NF-ΚB通路 预后 儿童 

分 类 号：R733.71[医药卫生—肿瘤]