Th9细胞及其细胞因子IL-9在慢性淋巴细胞白血病中的表达及临床意义  

Expression and Clinical Significance of Helper T Cells 9 and Its Sytokines Interleukin 9 in Chronic Lymphocytic Leukemia

作　　者：沙巴艾提·吐达洪 庞楠楠 谢仁古丽·阿力木 那扎凯提·依明 郭沙 张冉冉 龚珊 曲建华[1] Shabaaiti Tudahong;PANG NanNan;Xierenguli Alimu;Nazhakaiti Yiming;GUO Sha;ZHANG RanRan;GONG Shan;QU JianHua(Hematologic Disease Center,The First Affiliated Hospital of Xinjiang Medical University,Xinjiang Uygur Autonomous Region Institute of Hematology,Urumqi 830054,Xinjiang Uygur Autonomous Region,China;Key Laboratory of Biomedical Technology,Suzhou Institute of Biomedical Engineering and Technology,Chinese Academy of Sciences,Suzhou 215163,Jiangsu Province,China)

机构地区：[1]新疆医科大学第一附属医院血液病中心,新疆维吾尔自治区血液病研究所,新疆乌鲁木齐830054 [2]中国科学院苏州生物医学工程技术研究所中国科学院生物医学检验技术重点实验室,江苏苏州215163

出　　处：《中国实验血液学杂志》2023年第6期1663-1669,共7页Journal of Experimental Hematology

基　　金：国家自然科学基金地区项目(82160034);自治区天山创新团队项目(2022D14008);中国博士后科学基金面上项目(2022M712331)。

摘　　要：目的:探讨辅助性T细胞9(Th9)比例及其细胞因子白介素9(IL-9)在慢性淋巴细胞白血病(CLL)患者外周血中的表达水平及临床意义。方法:选取2021年6月至2022年6月于新疆医科大学第一附属医院就诊的43例初诊CLL患者作为病例组,采用Binet分期系统将病例组分为Binet A组(13例)、Binet B组(20例)和Binet C组(10例),同期在本院体检的20例健康志愿者作为对照组。流式细胞术检测外周血Th9细胞比例,Western blot检测Th9特异性转录因子PU.1和IRF4的表达水平,ELISA检测血清细胞因子IL-9的表达水平。比较各组Th9细胞比例、PU.1、IRF4和IL-9的表达差异;结合患者临床资料分析Th9比例、IL-9与CLL临床病理指标的相关性。结果:CLL组Th9细胞比例、PU.1、IRF4和IL-9的表达均明显高于对照组(P<0.05);组内比较,CLL患者Binet B和C组的Th9细胞比例和IL-9的表达均高于Binet A组(P<0.05),Th9细胞比例在Binet B组与C组间差异无统计学意义(P> 0.05),IL-9在Binet C组中的表达明显高于Binet B组(P<0.05);Th9细胞、IL-9在β2微球蛋白异常、IGHV未突变、TP53异常和肝脾淋巴结肿大患者中高表达,IL-9在肝肿大患者中高表达,而与年龄、性别等因素无关(P> 0.05)。Spearman相关性分析结果显示,CLL患者Th9细胞比例与淋巴细胞计数、淋巴细胞比例呈负相关(rs=-0.32,rs=-0.34),Th9比例、IL-9与Binet分期、Rai分期和CLL-IPI评分呈正相关(rs=0.79,rs=0.54,rs=0.58;rs=0.72,rs=0.63,rs=0.45),而与WBC、CD4+T细胞、CD8+T细胞等因素无关(P> 0.05),Th9比例与IL-9呈正相关(rs=0.53)。结论:Th9细胞和IL-9在CLL中异常高表达,并与CLL不良预后相关。Objective:To investigate the expression and clinical significance of T helper cell9(Th9)and its cytokine interleukin9(IL-9)in peripheral blood of patients with chronic lymphocytic leukemia(CLL).Methods:A total of43newly diagnosed patients with chronic lymphocytic leukemia in the First Affiliated Hospital of Xinjiang Medical University from June2021to June2022were selected as the case group.The patients were divided into Binet A group(13cases),Binet B group(20cases)and Binet C group(10cases)by Binet staging system,and20healthy volunteers who underwent physical examinationin in our hospital in the same period served as control group.The proportion of Th9cells in peripheral blood was detected by flow cytometry,the expression level of Th9specific transcription factors PU.1and IRF4was detected by Western blot,and the expression level of serum cytokine IL-9was detected by ELISA.The proportion of Th9,the expression of PU.1,IRF4and IL-9in each group were compared,and the correlation between the proportion of Th9,IL-9and clinicopathological indexes of CLL patients was analyzed.Results:The proportion of Th9,the expression of PU.1,IRF4and IL-9in CLL group were significantly higher than those in control group(P<0.05),the proportion of Th9and the expression of IL-9in Binet B and C group were higher than those in Binet A group(P<0.05),but there was no significant difference in the proportion of Th9cells between Binet B group and C group(P>0.05).The expression of IL-9in Binet C group was significantly higher than that in Binet B group(P<0.05).The proportion of Th9cells and IL-9were highly expression in patients withβ2microglobulin abnormality,IGHV unmutation,P53abnormality and hepatosplenic lymph node enlargement(P<0.05),but not related to age and sex(P>0.05).The results of Spearman correlation analysis showed that the proportion of Th9in patients with CLL was negatively correlated with the lymphocytic account and lymphocyte proportion(rs=-0.32,rs =-0.34).The proportion of Th9and IL-9were positively correlated with Binet stage

关 键 词：慢性淋巴细胞白血病 辅助性T细胞9 白介素9 预后 

分 类 号：R733.72[医药卫生—肿瘤]