伊藤杂种茎叶化学成分及其综合抗氧化能力  

Chemical Composition and Antioxidant Activity in Stems and Leaves of Paeonia Intersubgeneric Hybrids

作　　者：陈友锋 佟宁宁 彭丽平 江莉娜 刘燕[1] 刘政安[2] CHEN Youfeng;TONG Ningning;PENG Liping;JIANG Li’na;LIU Yan;LIU Zheng’an(Beijing Key Laboratory of Ornamental Plants Germplasm Innovation&Molecular Breeding/National Engineering Research Center for Floriculture/Beijing Laboratory of Urban and Rural Ecological Environment/Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education College of Landscape Architecture,Beijing Forestry University,Beijing 100083,China;Key Laboratory of Plant Resources/China National Botanical Garden,Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China;College of Horticulture,Hunan Agricultural University,Changsha,Hunan 410128,China)

机构地区：[1]花卉种质创新与分子育种北京市重点实验室/国家花卉工程技术研究中心/城乡生态环境北京实验室/林木花卉遗传育种教育部重点实验室/北京林业大学园林学院,北京100083 [2]中国科学院植物研究所北方资源植物重点实验室/国家植物园,北京100093 [3]湖南农业大学园艺学院,湖南长沙410128

出　　处：《福建农业学报》2023年第8期932-943,共12页Fujian Journal of Agricultural Sciences

基　　金：中国科学院战略性科技先导科技专项子课题(XDA23080601)。

摘　　要：【目的】探究芍药属亚属间杂种(伊藤杂种)的茎叶药用价值。【方法】利用高效液相色谱-二极管阵列检测(HPLC-DAD)与高效液相色谱-四级杆-飞行时间串联质谱技术(HPLC-Q-TOF-MS)对16个伊藤杂种茎、叶的次生代谢物进行定性、定量分析,并对其总酚含量和体外抗氧化活性进行测定。【结果】在16个伊藤杂种茎叶中共检测到19种次生代谢物,分别属于单萜苷类(5种)、黄酮类(6种)、单宁类(4种)、酚类(4种)。16个伊藤杂种茎叶中的代谢物成分基本相同;除酚类外,叶中其他3类代谢物和总代谢物含量均显著高于茎。16个伊藤杂种叶的抗氧化能力均显著高于茎;5个品种叶中的芍药苷含量高于药典规定的最低标准(18 mg·g^(−1))。聚类分析结果显示,16个品种被聚为3类,其中第3类群包含2个品种,特点为叶中除单萜苷类含量中等外,各项指标均最高。【结论】伊藤杂种的茎叶具有潜在的药用价值,筛选出2个具有药用植物潜力的伊藤杂种Unique和Lollipop。【Objective】Pharmaceutical property of the stems and leaves of Paeonia intersubgeneric hybrids(PIHs)was evaluated.【Method】Secondary metabolites in 16 PIHs were extracted from the stems and leaves and analyzed by the highperformance liquid chromatography with a diode array detector(HPLC-DAD)and the high-performance liquid chromatography quadrupole time-of-fight mass spectrometry(HPLC-Q-TOF-MS).Total phenolic content and in vitro antioxidant activity of the extract were measured.【Results】(1)Nineteen metabolites were identified from the extracts that included 5 monoterpene glycosides,6 flavonoids,4 tannins,and 4 phenols.(2)The compositions of all tested hybrids were essentially identical.(3)Except for phenols,the content of individual group or total amount of compounds in the 3 groups were significantly higher in the leaves than in the stems.(4)The in vitro antioxidant activity was tested significantly greater in the leaves than in the stems as well.(5)The contents of paeoniflorin in the leaves of 5 varieties were above the minimum 18 mg·g^(−1) as stipulated by the pharmacopoeia.(6)A cluster analysis grouped the 16 cultivars into 3 categories with one that contained two varieties,which scored the highest on all indicators except being moderate on monoterpene glycosides.【Conclusion】The stems and leaves of“Unique”and“Lollipop”in the 16 tested PIHs appeared to have pharmaceutical values.

关 键 词：芍药属 亚属间杂交种 药用成分 抗氧化能力 芍药苷 

分 类 号：S682[农业科学—观赏园艺]