miR-429在上皮性卵巢癌进展中的作用及机制研究  

Role and mechanism of miR-429 in the progression of epithelial ovarian cancer

作　　者：赵平鸽 张亚楠 张文雅 徐鹏[1] 黄敏[4] 李朦 滕佳茜 匡野[1] ZHAO Pingge;ZHANG Yanan;ZHANG Wenya;XU Peng;HUANG Min;LI Meng;TENG Jiaqian;KUANG Ye(Department of Obstetrics and Gynecology,the Second Affiliated Hospital of Harbin Medical University,Harbin,Heilongjiang 150086,China)

机构地区：[1]哈尔滨医科大学附属第二医院妇产科,黑龙江哈尔滨150086 [2]河北北方学院附属第一医院妇产科,河北张家口050051 [3]沧州市中心医院妇产科,河北沧州061011 [4]哈尔滨医科大学医学遗传学研究室,黑龙江哈尔滨150081

出　　处：《中华全科医学》2023年第10期1661-1664,共4页Chinese Journal of General Practice

基　　金：黑龙江省自然科学基金项目(LH2021H034)。

摘　　要：目的 探讨miR-429在上皮性卵巢癌组织中的表达及其与卵巢癌细胞增殖和迁移的关系,寻找其下游靶基因,并探讨miR-429靶基因DNAJB6在卵巢癌中的作用。方法 收集2019年9月—2022年2月期间于哈尔滨医科大学附属第二医院经妇科手术切除得到的27例上皮性卵巢癌组织和14例正常卵巢组织,使用实时荧光定量聚合酶链反应检测组织中miR-429及DNAJB6 mRNA的表达。在人卵巢癌细胞株A2780中敲低miR-429,使用CCK-8、平板克隆形成及Transwell细胞迁移实验在体外检测肿瘤细胞增殖及迁移能力的变化。采用生物信息学软件预测miR-429下游靶基因并分析其在卵巢癌中的预后价值,应用qRT-PCR和Western blotting实验进一步证实miR-429与DNAJB6之间的调控关系。结果 与正常卵巢组织[0.886(0.785,2.014)]相比,miR-429在上皮性卵巢癌组织[2.048(0.830,5.281)]中高表达(P=0.018),过表达miR-429可以增强卵巢癌细胞的增殖和迁移能力。与正常卵巢组织(1.290±0.781)相比,DNAJB6在上皮性卵巢癌组织(0.710±0.456)中低表达(P=0.004),与卵巢癌患者的预后关系密切。相对于对照组DNAJB6 mRNA的表达量(1.020±0.251),敲低miR-429可以上调DNAJB6 mRNA的表达量(2.839±0.305,P=0.001);敲低miR-429同样也上调了DNAJB6蛋白的表达(P=0.049)。结论 miR-429在上皮性卵巢癌中发挥促癌作用,可以调控靶基因DNAJB6的表达,提示miR-429/DNAJB6可能是卵巢癌治疗的新靶点。Objective To investigate the expression of miR-429 in epithelial ovarian cancer tissues and its relationship with the proliferation and migration of ovarian cancer cells,and to explore the role of miR-429 target gene DNAJB6 in ovarian cancer.Methods Twenty-seven epithelial ovarian cancer tissues and fourteen normal ovarian tissues obtained by gynecological surgical resection at the Second Affiliated Hospital of Harbin Medical University during the period from September 2019 to February 2022 were selected to detect the expression of miR-429 and DNAJB6 mRNA in the tissues using real-time fluorescence quantitative polymerase chain reaction(RT-qPCR).In human ovarian cancer cell line A2780,miR-429 was knocked down and changes in tumor cell proliferation and migration ability were measured in vitro by using CCK-8 kit,plate clone formation and Transwell cell migration assay.The downstream target genes of miR-429 were predicted by bioinformatics software and its prognostic value in ovarian cancer was analyzed.RT-qPCR and Western blot assays were applied to further confirm the regulatory relationship between miR-429 and DNAJB6.Results Compared with normal ovarian tissues 0.886(0.785,2.014),miR-429 was highly expressed in epithelial ovarian cancer tissues 2.048(0.830,5.281),P=0.018,and overexpression of miR-429 could enhance the proliferation and migration of ovarian cancer cells.Compared with normal ovarian tissues(1.290±0.781),DNAJB6 was lowly expressed in epithelial ovarian cancer tissues(0.710±0.456,P=0.004),which was closely related to the prognosis of ovarian cancer patients.Knockdown of miR-429 upregulated DNAJB6 mRNA expression(2.839±0.305,P=0.001)compared to the control(1.020±0.251);knockdown of miR-429 also upregulated DNAJB6 protein expression(P=0.049).Conclusion miR-429 plays a pro-cancer role in epithelial ovarian cancer and can regulate the expression of the target gene DNAJB6,suggesting that miR-429/DNAJB6 may be a new target for ovarian cancer therapy.

关 键 词：上皮性卵巢癌 微小RNA-429 增殖 迁移 

分 类 号：R737.31[医药卫生—肿瘤] R730[医药卫生—临床医学]