丙戊酸协同顺铂抑制乳腺癌和结直肠癌细胞生长  

Valproic Acid and Cisplatin Inhibit the Growth of Breast Cancer and Colorectal Cancer Cells

作　　者：成含月 杨怡静 张亚丽 王涛 李羚 王彩云 徐璐 CHENG Han-yue;YANG Yi-jing;ZHANG Ya-li;WANG Tao;LI Ling;WANG Cai-yun;XU Lu(Department of Pharmacology and Chemical Biology,Shanghai Jiao Tong University School of Medicine,Shanghai,200025,China)

机构地区：[1]上海交通大学医学院药理学与化学生物学系,上海200025

出　　处：《现代生物医学进展》2023年第19期3601-3605,3624,共6页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81372522,82073868)。

摘　　要：目的:探究丙戊酸(Valproic acid,VPA)协同顺铂抑制乳腺癌和结直肠癌细胞增殖。方法:首先使用Western blot检测VPA对Acetyl-Histone H3蛋白水平的影响,使用Cell Counting Kit-8(CCK-8)法检测VPA对乳腺癌和结直肠癌细胞的细胞活力的影响。其次单药顺铂、VPA和联合用药处理乳腺癌细胞MDA-MB-231和结直肠癌细胞HCT-15,使用IncuCyte动态检测细胞生长过程和生长终点。结果:发现VPA可抑制组蛋白去乙酰化酶的功能,升高Acetyl-Histone H3的蛋白水平,VPA可抑制乳腺癌细胞和结直肠癌细胞增殖,且对VPA的药物敏感性相似;顺铂和VPA连用后可显著抑制乳腺癌和结直肠癌细胞增殖和活力。结论:本文发现VPA抑制组蛋白去乙酰化酶发挥抑制乳腺癌和结直肠癌细胞生长的新机制,并可以与顺铂连用提高抗肿瘤效果和药物敏感性,为同时患有癫痫和肿瘤的人群提供新的治疗思路。Objective:To explore the inhibitory effect of Valproic acid(VPA)and cisplatin on the proliferation of breast cancer and colorectal cancer cells.Methods:Firstly,Western blot was used to detect the effects of VPA on Acetyl-Histone H3 protein levels,and Cell Counting Kit-8(CCK-8)was used to detect the effects of VPA on the cell viability of breast cancer and colorectal cancer cells.Secondly,breast cancer cells MDA-MB-231 and colorectal cancer cells HCT-15 were treated with single drug cisplatin,VPA and a combination of drugs.IncuCyte was used to dynamically detect the cell growth process and growth endpoint.Results:It was found that VPA could inhibit the function of histone deacetylase and increase the Acetyl-Histone H3 protein level.VPA could inhibit the proliferation of breast cancer cells and colorectal cancer cells,and the drug sensitivity to VPA was similar.The combination of cisplatin and VPA can significantly inhibit the proliferation and viability of breast cancer and colorectal cancer cells.Conclusions:This study found that VPA inhibits histone deacetylase to play a new role in inhibiting the growth of breast cancer and colorectal cancer cells,and can be combined with cisplatin to improve the antitumor effect and drug sensitivity,providing a new therapeutic idea for patients with epilepsy and tumor.

关 键 词：乳腺癌 结直肠癌 丙戊酸 顺铂 

分 类 号：R-33[医药卫生] R735.3R737.9