肝细胞癌组织miR-124-3p、miR-212-5p表达与PI3K/Akt信号通路和预后的关系研究  

Relationship between the Expression of miR-124-3p and miR-212-5p in Hepatocellular Carcinoma Tissue and the PI3K/Akt Signaling Pathway and Prognosis

作　　者：张泽宇 谢潭 王飞通 魏鑫 刘斌 ZHANG Ze-yu;XIE Tan;WANG Fei-tong;WEI Xin;LIU Bin(Department of Hepatobiliary Surgery,Xuzhou Medical University Affiliated Hospital,Xuzhou,Jiangsu,221000,China)

机构地区：[1]徐州医科大学附属医院肝胆外科,江苏徐州221000

出　　处：《现代生物医学进展》2023年第19期3625-3629,共5页Progress in Modern Biomedicine

基　　金：江苏省高等学校自然科学研究面上项目(17KJD320004)。

摘　　要：目的:探讨肝细胞癌组织中微小核糖核酸(miR)-124-3p、miR-212-5p表达与磷脂酰肌醇3-激酶(PI3K)/蛋白激酶B(Akt)信号通路和预后的关系。方法:选择2017年9月至2019年9月徐州医科大学附属医院肝胆外科收治的93例肝细胞癌患者,采用实时荧光定量聚合酶链反应(qRT-PCR)检测肝细胞癌组织中miR-124-3p、miR-212-5p、PI3K信使RNA(mRNA)、Akt mRNA的表达。Pearson相关性分析miR-124-3p、miR-212-5p表达与PI3K/Akt信号通路相关mRNA表达的相关性。绘制Kaplan-Meier生存曲线,Log-Rank检验不同miR-124-3p、miR-212-5p表达肝细胞癌患者3年总生存率(OS)的差异。结果:肝细胞癌组织中miR-124-3p表达低于癌旁组织(P<0.05),miR-212-5p、PI3K mRNA、Akt mRNA表达高于癌旁组织(P<0.05)。肝细胞癌组织中miR-124-3p表达与PI3K mRNA、Akt mRNA表达呈负相关(P<0.05),miR-212-5p表达与PI3K mRNA、Akt mRNA表达呈正相关(P<0.05)。Ⅱa期、低分化患者肝细胞癌组织中miR-124-3p表达低于Ⅰa-Ⅰb期、中高分化患者(P<0.05),miR-212-5p表达高于Ⅰa-Ⅰb期、中高分化患者(P<0.05)。随访期间死亡37例,miR-124-3p低表达组3年OS为42.55%,低于miR-124-3p高表达组的78.26%(P<0.05),miR-212-5p高表达组3年OS为50.00%,低于miR-212-5p低表达组的71.11%(P<0.05)。结论:肝细胞癌组织中miR-124-3p表达下调,miR-212-5p表达上调,且与肝细胞癌PI3K/Akt信号通路激活,PI3K mRNA和Akt mRNA高表达,低分化,CNLC分期Ⅱa期以及低OS有关。Objective:To investigate the relationship between the expression of microribonucleic acid(miR)-124-3p and miR-212-5p in hepatocellular carcinoma tissues and the phosphatidylinositol 3-kinase(PI3K)/protein kinase B(Akt)signaling pathway and prognosis.Methods:93 patients with hepatocellular carcinoma who were admitted to the Department of Hepatobiliary Surgery of Xuzhou Medical University Affiliated Hospital were selected from September 2017 to September 2019,the expression of miR-124-3p,miR-212-5p,PI3K messenger RNA(mRNA),Akt mRNA in hepatocellular carcinoma tissue was detected by real-time quantitative polymerase chain reaction(qRT-PCR).The correlation between miR-124-3p,miR-212-5p expression and PI3K/Akt signaling pathway related mRNA expression were analyzed by Pearson correlation.Drawed Kaplan-Meier survival curve and Log-Rank test for differences in 3-year overall survival rate(OS)of hepatocellular carcinoma patients with different miR-124-3p and miR-212-5p expression levels.Results:The expression of miR-124-3p in hepatocellular carcinoma tissue was lower than that in adjacent tissue(P<0.05),while the expression of miR-212-5p,PI3K mRNA,and Akt mRNA were higher than those in adjacent tissue(P<0.05).The expression of miR-124-3p in hepatocellular carcinoma tissue was negatively correlated with the expression of PI3K mRNA and Akt mRNA(P<0.05),while the expression of miR-212-5p was positively correlated with the expression of PI3K mRNA and Akt mRNA(P<0.05).The expression of miR-124-3p in hepatocellular carcinoma tissues of stage II a and poorly differentiated patients were lower than those of stageⅠa-Ⅰb and moderately well differentiated patients(P<0.05),while the expression of miR-212-5p were higher than those of stageⅠa-Ⅰb and moderately well differentiated patients(P<0.05).During the follow-up period,37 deaths occurred.The 3-year OS in the low expression group of miR-124-3p was 42.55%,lower than 78.26%in the high expression group of miR-124-3p(P<0.05).The 3-year OS in the high expression group of miR-21

关 键 词：肝细胞癌 PI3K/AKT信号通路 miR-124-3p miR-212-5p 预后 

分 类 号：R-33[医药卫生] R735.7