宫颈癌组织miR-200b-5p、miR-424-5p表达与临床病理特征、PI3K/AKT/mTOR信号通路和预后的关系研究  

Relationship Study between miR-200b-5p and miR-424-5p Expression in Cervical Cancer Tissues and Clinicopathological Features,PI3K/AKT/mTOR Signaling Pathway and Prognosis

作　　者：王燕[1] 高婉 王聪 李亚萍[1] 李冰琳[1] 张淑会 WANG Yan;GAO Wan;WANG Cong;LI Ya-ping;LI Bing-lin;ZHANG Shu-hui(Department of Obstetrics and Gynecology,Xi'an Central Hospital,Xi'an,Shaanxi,710003,China;Department of Obstetrics and Gynecology,Pucheng County Hospital of Shaanxi Province,Weinan,Shaanxi,715500,China)

机构地区：[1]西安市中心医院妇产科,陕西西安710003 [2]陕西省蒲城县医院妇产科,陕西渭南715500

出　　处：《现代生物医学进展》2023年第19期3714-3718,共5页Progress in Modern Biomedicine

基　　金：陕西省自然科学基金项目(2121JM-563)。

摘　　要：目的:探讨宫颈癌组织微小核糖核酸(miRNA)-200b-5p、miR-424-5p表达与临床病理特征、磷脂酰肌醇3-激酶/蛋白激酶B/哺乳动物雷帕霉素靶蛋白(PI3K/AKT/mTOR)信号通路和预后的关系。方法:选取2016年7月~2019年6月西安市中心医院收治的123例宫颈癌患者,采用定量聚合酶链式反应(qPCR)检测癌组织与癌旁组织中miR-200b-5p、miR-424-5p、PI3K信使RNA(mRNA)、AKT mRNA、mTOR mRNA表达。分析miR-200b-5p、miR-424-5p表达与PI3K mRNA、AKT mRNA、mTOR mRNA表达的相关性及与临床病理特征的关系。采用K-M法绘制不同miR-200b-5p、miR-424-5p表达宫颈癌患者生存曲线。结果:与癌旁组织比较,宫颈癌组织中miR-200b-5p、miR-424-5p表达降低,PI3K mRNA、AKT mRNA、mTOR mRNA表达升高(P<0.05)。Pearson相关性分析显示,宫颈癌组织中miR-200b-5p、miR-424-5p表达与PI3K mRNA、AKT mRNA、mTOR mRNA表达均呈负相关,PI3K mRNA、AKT mRNA、mTOR mRNA表达呈两两相关(P<0.05)。miR-200b-5p、miR-424-5p表达与宫颈癌分化程度、国际妇产科联盟(FIGO)分期和淋巴结转移有关(P<0.05)。随访3年,123例宫颈癌患者累积生存率为62.60%(77/123)。K-M生存曲线分析显示,miR-200b-5p、miR-424-5p高表达组累积生存率分别高于miR-200b-5p、miR-424-5p低表达组(P<0.05)。结论:宫颈癌组织中miR-200b-5p、miR-424-5p低表达,与分化程度、FIGO分期、淋巴结转移、PI3K/AKT/mTOR信号通路和预后有关。Objective:To investigate the relationship between microribonucleic acid(miRNA)-200b-5p and miR-424-5p expres-sion in cervical cancer tissues and clinicopathological features,phosphatidylinositol 3-kinase/protein kinase B/mammalian target of rapamycin(PI3K/AKT/mTOR)signaling pathway and prognosis.Methods:123 patients with cervical cancer who were admitted to Xi'an Central Hospital from July 2016 to June 2019 were selected.Quantitative polymerase chain reaction(qPCR)was used to detect the expressions of miR-200b-5p,miR-424-5p,PI3K messenger RNA(mRNA),AKT mRNA and mTOR mRNA in cancer tissues and para-cancer tissues.The correlation between the expressions of miR-200b-5p and miR-424-5p and the expressions of PI3K mRNA,AKT mRNA and mTOR mRNA and their relationship with clinicopathological features were analyzed.Survival curves of patients with cervical cancer with different expressions of miR-200b-5p and miR-424-5p were plotted by K-M method.Results:Compared with paracancer tissues,the expressions of miR-200b-5p and miR-424-5p in cervical cancer tissues were decreased,while the expressions of PI3K mRNA,AKT mRNA and mTOR mRNA were increased(P<0.05).Pearson correlation analysis showed that the expressions of miR-200b-5p and miR-424-5p in cervical cancer tissues were negatively correlated with the expressions of PI3K mRNA,AKT mRNA and mTOR mRNA,the expressions of PI3K mRNA,AKT mRNA and mTOR mRNA were pairwise correlated(P<0.05).The expressions of miR-200b-5p and miR-424-5p were correlated with the degree of cervical cancer differentiation,International Federation of Gynecology and Obstetrics(FIGO)staging and lymph node metastasis(P<0.05).Following up for 3 years,the cumulative survival rate of 123 patients with cervical cancer was 62.60%(77/123).K-M survival curve analysis showed that the cumulative survival rate of miR-200b-5p and miR-424-5p high-expression groups were higher than that of miR-200b-5p and miR-424-5p low-expression groups,respectively(P<0.05).Conclusion:The low expression of miR-200b-5p and miR-424-5p in c

关 键 词：宫颈癌 miR-200b-5p miR-424-5p 病理特征 PI3K/AKT/mTOR信号通路 预后 

分 类 号：R737.33[医药卫生—肿瘤]