Inhibitory Effect of Jingui Shenqi Pills on Glucocorticoid-Enhanced Axial Length Elongation in Experimentally Myopic Guinea Pigs  

作　　者：JIANG Qian JIANG Wen-jun YANG Cheng-xiu ZHANG Rui-xue SUN Wei GUO Da-dong WU Jiang-feng GUO Bin WANG Xing-rong BI Hong-sheng 

机构地区：[1]Department of Ophthalmology and Optometry,Shandong University of Traditional Chinese Medicine,Jinan(250014),China [2]Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine,Jinan(250002),China [3]Shandong Province Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Disease,Shandong Academy of Eye Disease Prevention and Therapy,Jinan(250002),China

出　　处：《Chinese Journal of Integrative Medicine》2023年第11期989-997,共9页中国结合医学杂志（英文版）

基　　金：Supported by the National Key Research and Development Program(Nos.2021YFC2702103,2019YFC1710204);the Key Research and Development Program of Shandong Province(No.2019GSF108252);the Natural Science Foundation of Shandong Province(No.ZR2020MH393)。

摘　　要：Objective:To explore the underlying mechanism of inhibition by Jingui Shenqi Pills(JGSQP)on glucocorticoid-enhanced axial length elongation in experimental lens-induced myopia(LIM)guinea pigs.Methods:Sixty 2-week old male guinea pigs were randomly divided into 4 groups with 15 guinea pigs in each group,according to the random numbers generated by SPSS software:control,LIM,saline and JGSQP groups.The control group includes animals with no treatment,while the guinea pigs in the other 3 groups received lens-induced myopization on the right eyes throughout the experiment(for 8 weeks).The saline and JGSQP groups were given daily intraperitoneal injections of 10 mg/kg hydrocortisone for 2 consecutive weeks at the same time,and then orally administered either saline or JGSQP 13.5 g/(kg·d)for 6 consecutive weeks.Body weight,anal temperature and animal appearance were observed and recorded to evaluate the GC-associated symptoms.The ocular parameters,including refraction and axial length,were measured by streak retinoscopy and A-scan ultrasonography,respectively.The levels of plasma hormones associated with the hypothalamic-pituitary-adrenal axis(HPAA),including free triiodothyronine,free thyroxine,estradiol and testosterone,were measured by radioimmunoassay,and cyclic adenosine monophosphate(cAMP)and cyclic guanosine monophosphate were measured by enzyme-linked immunosorbent assay.In addition,the mRNA and protein expressions of retinal amphiregulin(AREG)was measured by quantitative real-time polymerase chain reaction and Western blotting,respectively.Results:JGSQP effectively increased body weight and anal temperature,improved animal appearance and suppressed axial length elongation in glucocorticoidenhanced myopic guinea pigs with normalization of 4 HPAA-associated plasma hormones(all P<0.05).The plasma level of cAMP was significantly increased,whereas the plasma level of cGMP and the mRNA and protein expressions of retinal AREG were decreased after treatment with JGSQP(all P<0.05).Conclusion:JGSQP exhibited a significa

关 键 词：GUINEA TREATMENT normalized 

分 类 号：R285.5[医药卫生—中药学]