Radix Scrophulariae Extracts Exert Effect on Hyperthyroidism via MST1/Hippo Signaling Pathway  

作　　者：ZHANG Ning YE Tao LU Xu LI Zi-hui LI Ling 

机构地区：[1]Department of Pharmacy,The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine,Guiyang(50001),China [2]Department of Chinese Medicine Rehabilitation,The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine,Guiyang(50001),China [3]College of Pharmacy,Guizhou University of Traditional Chinese Medicine,Guiyang(550025),China [4]College of Life and Health,Dalian University,Dalian,Liaoning Province(116622),China

出　　处：《Chinese Journal of Integrative Medicine》2023年第11期998-1006,共9页中国结合医学杂志（英文版）

基　　金：Supported by the National Natural Science Foundation of China(No.82060830);Guizhou Province Traditional Chinese Medicine,Ethnic Medicine Science,and Technology Research Poject(No.QZYY-2021-113);Science and Technology Fund Project of Guizhou Provincial Health Commission(No.gzwkj2021-457)。

摘　　要：Objective:To explore the mechanism of Radix Scrophulariae(RS)extracts in the treatment of hyperthyroidism rats by regulating proliferation,apoptosis,and autophagy of thyroid cell through the mammalian sterile 20-like kinase 1(MST1)/Hippo pathway.Methods:Twenty-four rats were randomly divided into 4 groups according to a random number table:control,model group,RS,and RS+Hippo inhibitor(XMU-MP-1)groups(n=6 per group).Rats were gavaged with levothyroxine sodium tablet suspension(LST,8μg/kg)for 21 days except for the control group.Afterwards,rats in the RS group were gavaged with RS extracts at the dose of 1,350 mg/kg,and rats in the RS+XMU-MP-1 group were gavaged with 1,350 mg/kg RS extracts and 1 mg/kg XMU-MP-1.After 15 days of administration,thyroid gland was taken for gross observation,and histopathological changes were observed by hematoxylin-eosin staining.The structure of Golgi secretory vesicles in thyroid tissues was observed by transmission electron microscopy.The expression of thyrotropin receptor(TSH-R)was observed by immunohistochemistry.Terminal-deoxynucleoitidyl transferase mediated nick end labeling assay was used to detect cell apoptosis in thyroid tissues.Real-time quantity primer chain reaction and Western blot were used to detect the expressions of MST1,p-large tumor suppressor gene 1(LATS1),p-Yes1 associated transcriptional regulator(YAP),proliferating cell nuclear antigen(PCNA),G_(1)/S-specific cyclin-D1(Cyclin D1),B-cell lymphoma-2(Bcl-2),Caspase-3,microtubule-associated proeins light chain 3Ⅱ/Ⅰ(LC3-Ⅱ/Ⅰ),and recombinant human autophagy related 5(ATG5).Thyroxine(T4)level was detected by enzyme-linked immunosorbent assay.Results:The thyroid volume of rats in the model group was significantly increased compared to the normal control group(P<0.01),and pathological changes such as uneven size of follicular epithelial cells,disorderly arrangement,and irregular morphology occurred.The secretion of small vesicles by Golgi apparatus was reduced,and the expressions of receptor protein TSH-R and

关 键 词：RADIX inhibited SCR 

分 类 号：R285.5[医药卫生—中药学]