A comparative study of two methods for establishing a chronic non‑bacterial prostatitis model in rats‑  

作　　者：TANG Yi-wen WANG Xiong ZHOU Yan-yan CHEN Hao-te ZHANG Ze-jia WANG Zhong GAO Qing-he LIU Jian-gang GAO Zhan 

机构地区：[1]Graduate School of Beijing University of Chinese Medicine,Beijing 100029,China [2]Department of Urology,Xiyuan Hospital,Chinese Academy of Traditional Chinese Medicine,Beijing 100091,China [3]Department of Male Medicine,Xiyuan Hospital,Chinese Academy of Traditional Chinese Medicine,Beijing 100091,China [4]Institute of Cardiovascular Diseases,Chinese Academy of Traditional Chinese Medicine,Beijing 100091,China

出　　处：《Journal of Hainan Medical University》2023年第13期1-7,共7页海南医学院学报（英文版）

基　　金：National Natural Science Foundation Project(82104881);Major Scientific and Technological Innovation Project of the China Academy of Chinese Medical Sciences(CI2021A02205)。

摘　　要：Objective:To compare the biological characteristics and stability of the chronic non-bacterial prostatitis(CNP)model in rats induced by the depot combined with estrogen induction method and the autoimmune response induction method.Methods:The CNP rat model was prepared using the depot combined with 17β-estradiol induction method in the depot hormone group and three concentrations of prostate protein homogenate at 40 mg/mL,20 mg/mL and 10 mg/mL in the autoimmune group,respectively.The degree of prostate tissue damage was evaluated by pathology(HE staining),and the immunoturbidimetric method was used to evaluate the contents of immunoglobulins IgA,IgM and IgG.ELISA was used to evaluate interleukin-1β(IL-1β),interleukin-10(IL-10),tumor necrosis factor-α(TNF-α)and high-sensitivity-C-reactive protein(hs-CRP),and electrochemiluminescence was used to evaluate the expression level of testosterone(T),and the two model methods were compared.Results:Compared with the sham-operated group,the body mass of rats in both models was significantly lower in the depot hormone group and autoimmune group before extraction(P<0.01,P<0.01),and histopathology of the prostate in both models showed destruction of glandular structure and a significant increase in inflammatory cells.Compared with the depot hormone group and the autoimmune group,the histopathological changes and inflammatory pathological scores of prostate and the contents of immune indexes IgA,IgM and IgG were significantly different in the depot hormone group(P<0.05,P<0.05,P<0.05),and the levels of IL-1β,IL-10,TNF-α,hs-CRP and T were significantly changed in the autoimmune group(P<0.01).The comparison between the high,medium and low dose groups of the autoimmune group,in which the pathological changes in the medium dose of the autoimmune group were slightly better than the other two groups between the groups,but the changes in IL-1β,IL-10,TNF-α,hs-CRP,and T in the low dose of the autoimmune group were the most significant(P<0.05).Conclusion:The pathological tissues

关 键 词：Chronic non‑bacterial prostatitis Debridement surgery AUTOIMMUNE Animal model RATS 

分 类 号：R69[医药卫生—泌尿科学]