光催化反应中活性氧物种产生及抗菌机制研究  

Generation and antimicrobial mechanisms of reactive oxygen species in photocatalysis

作　　者：王鹏鸽 张静 王震宇 赵宇蕾 黄宇[1] WANG Pengge;ZHANG Jing;WANG Zhenyu;ZHAO Yulei;HUANG Yu(Key Laboratory of Aerosol Chemistry and Physics,State Key Laboratory of Loess and Quaternary Geology,Institute of Earth Environment,Chinese Academy of Sciences,Xi’an 710061,China;Xi’an Institute for Innovative Earth Environment Research,Xi’an 710061,China)

机构地区：[1]中国科学院地球环境研究所黄土与第四纪地质国家重点实验室,中国科学院气溶胶化学与物理重点实验室,西安710061 [2]西安地球环境创新研究院,西安710061

出　　处：《地球环境学报》2023年第5期539-556,共18页Journal of Earth Environment

基　　金：国家重点研发计划(2017YFC0212200)。

摘　　要：空气中的致病菌引发的流行性疾病严重威胁人类生命安全。近年来,光催化微生物灭活技术作为一种广谱高效、安全稳定、持久耐热、不易产生耐药性、杀菌彻底的方法受到广泛关注。光催化反应中产生的活性氧物种(reactive oxygen species,ROS)在光催化抗菌中发挥着不可替代的作用,但特定类别ROS的产生和杀菌机制的研究较少,尚未有综述对其进行系统概述。本文重点从光催化半导体的能带结构与特定ROS(·O_(2)^(-)、·OH、H_(2)O_(2))氧化还原电势的关系综述了三种自由基的产生机制,从氧化能力、存在寿命、主要作用对象等方面简要讨论了自由基对细菌造成氧化损伤和功能失调的具体过程,另外还涉及ROS的检测方法和抗菌性能评价方式,进一步对光催化抗菌技术在环境消杀领域的应用前景进行了展望。Background,aim,and scope Epidemic diseases caused by bacterial and viral infections remain a growing health threat to people around the world.Compared to traditional disinfection techniques,photocatalysis has drawn tremendous attention for its high efficiency,good biocompatibility,little resistance,and broad-spectrum antibacterial performance.Oxidative stress induced by reactive oxygen species(ROS)in a photocatalytic process was considered as major antibacterial mechanism.While,the generation and disinfection mechanisms of ROS in photocatalysis have not been clearly elucidated yet at the molecular level.In this review,we surveyed on the processes of mainly forms of ROS generation,oxidative damage and dysfunction in biological systems.Based on the present reviews,the generation process,detection methods,antibacterial mechanisms of the three major ROS and evaluation methods on antibacterial performance in photocatalysis were summarized.Materials and methods This review discussed the generation and detection mechanisms of the individual ROS including superoxide(∙O),hydroxyl radical(∙OH)and hydrogen peroxide(H_(2)O_(2))in some typical photocatalytic antibacterial nanomaterials,analyzed the main processes of biological damages caused by ROS in photocatalysis disinfection,and evaluated the antibacterial effects through different antibacterial mechanisms.Results(1)Induced by solar irradiation,e−and h+migrated on the surfaces of photocatalysts possess reduction and oxidation properties,respectively,which can react with absorbed O_(2) and H_(2)O/OH−to produce∙O,∙OH,H_(2)O_(2) and etc.The absorbed O_(2) is reduced to generate∙O,H_(2)O_(2),and∙OH;H_(2)O is oxidized to produce∙O,∙OH,and H_(2)O_(2).(_(2))The ROS generated in photocatalysis are mainly detected by vibration spectroscopy,UV-visible spectrophotometry,and fluorescence emission technology.(3)Oxidative stress induced by ROS generated in photocatalysis is considered as one of the major mechanisms for photoinduced sterilization.All ROS,including∙

关 键 词：光催化 ROS 产生机制 检测技术 抗菌机理 

分 类 号：TU834.8[建筑科学—供热、供燃气、通风及空调工程]