HPLC法测定甘草养生酒中甘草酸含量的研究  

Determination Glycyrrhizic Acid Content in Glycyrrhiza Healthy Liquor by HPLC

作　　者：王丹 吴小娟 吉喆 兰余 吴卫宇 匡立华 周成坪 罗爽 杨洁 刘小刚[1,3] WANG Dan;WU Xiaojuan;JI Zhe;LAN Yu;WU Weiyu;KUANG Lihua;ZHOU Chengping;LUO Shuang;YANG Jie;LIU Xiaogang(Luzhou Laojiao Co.,Ltd.,Luzhou Sichuan 646000,China;Sichuan Guojian Inspection Co.,Ltd.,Luzhou Sichuan 646000,China;Luzhou Laojiao Health Liquor Co.,Ltd.,Luzhou Sichuan 646000,China)

机构地区：[1]泸州老窖股份有限公司,四川泸州646000 [2]四川国检检测有限责任公司,四川泸州646000 [3]泸州老窖养生酒业有限责任公司,四川泸州646000

出　　处：《食品与发酵科技》2023年第5期125-129,147,共6页Food and Fermentation Science & Technology

基　　金：四川省科技项目(2021ZYD0102)。

摘　　要：建立了甘草养生酒中甘草酸的高效液相色谱测定方法。不同甘草酸含量的养生酒样品经适当稀释或100℃水浴去除乙醇,定容后,以乙腈-0.05%磷酸水溶液为流动相,Athena C18-WP水相柱(4.6mm×250mm,5μm)分离的高效液相色谱法测定甘草酸含量。结果显示:该方法在2.0~400mg/L浓度范围内线性关系良好,相关系数R2为0.9994,线性方程为y=5.3538x-6.0226,方法检出限、定量限分别为0.50mg/kg、1.50mg/kg;仪器精密度良好,RSD为0.34%(n=6);甘草养生酒中4个加标水平下的回收率范围为90.3%~104.8%(n=6),相对标准偏差为1.748%~5.117%。该方法灵敏度高、分析时间短、稳定性好、准确可靠,是测定甘草养生酒中甘草酸含量的有效方法。To establish a HPLC method for the detection of glycyrrhizic acid content in glycyrrhiza healthy liquor.In the method,the experimental samples with different concentration levels of glycyrrhizic acid were measured after appropriate dilution or water bath at 100℃proposed to remove ethanol and dissolved with methanol.The analysts were separated on an Athena C18-WP column(4.6 mm×250 mm,5μm)by gradient elution with mixtures of acetonitrile-0.05%phosphoric acid solution.The results showed that the method had a good linear relationship in the range of 2.0~400 mg/L,with the correlation coefficient of 0.9994(y=5.3538x-6.0226).The limit of detection and limit of quantification were 0.50 mg/kg and 1.50 mg/kg.The instrument had a good precision,The RSD value of the peak areas was 0.34%(n=6).The recoveries of four spiking levels ranged from 90.3%to 104.8%(n=6).The RSD values of glycyrrhizic acid content ranged from 1.748%to 5.117%.The method has advantages such as high sensitivity,short analysis time,good stability and the results were accurate and reliable.It can be used to determine the content of glycyrrhizic acid in glycyrrhiza healthy liquor.

关 键 词：高效液相色谱法 甘草酸 甘草养生酒 

分 类 号：TS207.3[轻工技术与工程—食品科学]