淫羊藿中朝藿苷A、B和C的酶转化及其稀有箭藿苷的制备  

Rare Epimedium-flavonoid preparation from epimedin A,B and C by enzymatic conversion

作　　者：彭磊 徐丝瑜 武博[1] 王政浩 鱼红闪[1] PENG Lei;XU Siyu;WU Bo;WANG Zhenghao;YU Hongshan(College of Biotechnology,Dalian Polytechnic University,Dalian 116034,China)

机构地区：[1]大连工业大学生物工程学院,辽宁大连116034

出　　处：《食品与发酵工业》2023年第21期95-100,共6页Food and Fermentation Industries

基　　金：国家高端外国专家项目(GDT20152100019)。

摘　　要：淫羊藿是一种草本药用植物,主要活性成分是黄酮类化合物,其中淫羊藿苷、朝藿苷A、B和C含量较高,稀有淫羊藿黄酮类化合物利用价值高却含量低。Aspergillis sp.y48菌所产生的淫羊藿黄酮苷酶,能水解朝藿苷A和C的7-O-Glc糖基,转化成箭藿苷A和C;能水解朝藿苷B的7-O-Glc糖基和3-O-糖基生成箭藿苷B、淫羊藿次苷II和淫羊藿苷元。该研究将朝藿苷A、C转化为纯度均为98%的箭藿苷A、C,其摩尔得率均为95%以上;将朝藿苷B转化为纯度83%的箭藿苷B;通过延长酶反应时间,将朝藿苷B制备为纯度98%的淫羊藿苷元,摩尔得率为93.8%。由此,把淫羊藿中含量较高的朝藿苷A、B和C,采用酶转化法成功地制备为箭藿苷等稀有淫羊藿黄酮类化合物。该方法流程简单、转化率高、成本低、产品纯度高,研究结果为淫羊藿黄酮类化合物的高效利用和稀有淫羊藿黄酮类化合物的生产提供了参考。Epimedium is a medicinal herb,and its main active ingredients are flavonoids,among which icariin,epimedin A,B,and C are high in content,while rare Epimedium flavonoids have high utilization value but low content.An Epimedium-flavonoid-glucosidase from Aspergillus sp.y48 strain can hydrolyze 7-O-Glc glycosyl of epimedin A and C,and convert them into sagittatoside A and C.It can also hydrolyze 7-O-Glc glycosyl and 3-O-glycosyl of epimedin B to generate icariin II and icariin.In this paper,epimedin A and C were converted into sagittatoside A and C with a purity of 98%,and the molar yields were above 95%.Using this enzyme,epimedin B was converted into sagittatoside B with a purity of 83%.The enzyme reaction time was prolonged to prepare icariin with a purity of 98%and a molar yield of 93.8%.Therefore,epimedin A,B,and C,which have high content in Epimedium,were successfully prepared into rare Epimedium flavonoids by enzymatic conversion.This method has the advantages of a simple process,high conversion rate,low cost,and high product purity.There are no other related reports until now.The results of this study provide methods for the efficient utilization of flavonoids in Epimedium and the production of rare Epimedium-flavonoids.

关 键 词：箭藿苷 朝藿苷 淫羊藿苷元 淫羊藿次苷 酶转化 

分 类 号：R284[医药卫生—中药学]