马铃薯抗寒性鉴定及筛选  

Identification and Selection for Cold Resistance of Potato

作　　者：舒启琼 罗小波 李飞 罗充[2] 冯文豪 陈明俊 曹贞菊 SHU Qiqiong;LUO Xiaobo;LI Fei;LUO Chong;FENG Wenhao;CHEN Mingjun;CAO Zhenju(Institute of Biotechnology,Guizhou Academy of Agricultural Sciences/Guizhou Branch of National Potato Improvement Center/Guizhou Potato Engineering Technology Research Center,Guiyang 550006,China;School of Life Sciences,Guizhou Normal University,Guiyang 550001,China;Guizhou Province Agricultural Technology Extension Station,Guiyang 550001,China)

机构地区：[1]贵州省农科院生物技术研究所/国家马铃薯改良中心贵州分中心/贵州省马铃薯工程技术研究中心,贵阳550006 [2]贵州师范大学生命科学学院,贵阳550001 [3]贵州省农作物技术推广总站,贵阳550001

出　　处：《种子》2023年第9期121-126,131,共7页Seed

基　　金：贵州省科技支撑计划子课题(黔科合支撑[2022]重点025-3号);贵州省科技支撑计划子课题(黔科合支撑[2022]重点026-3号);国家马铃薯产业技术体系贵阳试验站(CARS-09-ES24)。

摘　　要：本研究以82份马铃薯材料为研究对象,采用电解质渗漏法测定离体叶片电导率,结合Logistic方程拟合求出半致死温度(LT_(50))进行抗寒性鉴定及筛选。结果发现,82份材料的LT 50值在-4.8℃~-1.6℃之间,聚类分析可将82份材料分为低温敏感型、中间型和较强抗寒型3类,筛选出大同20和Cip43等7份抗寒材料可作为马铃薯抗寒性后续研究材料。In this study,82 potato materials were used as research objects,and electrolyte leakage method was used to measure the electrical conductivity of leaves in vitro,and the half-lethal temperature(LT _(50))was obtained by Logistic equation fitting,so as to identify and screen the cold resistance.The results showed that the LT 50 value ranged from-4.8℃to-1.6℃.The 82 materials were divided into three types by cluster analysis,namely,low temperature sensitive type,intermediate type and strong cold resistance type.Seven cold-resistant materials,including Datong20 and Cip43,were screened out,which could provide reference materials for the follow-up study of potato cold resistance.

关 键 词：马铃薯 电解质渗漏法 半致死温度 抗寒性鉴定 

分 类 号：S532[农业科学—作物学]