基于BALB/c小鼠模型的松茸低温水提物的免疫调节作用  

Immunomodulatory Effect of Tricholoma matsutake Low-temperature Water ExtractsBased on BALB/c Mouse Model

作　　者：杜汉廷 林松毅 李冬梅[1] 葛麒 陈冬 Du Hanting;Lin Songyi;Li Dongmei;Ge Qi;Chen Dong(School of Food Science and Technology,Dalian Polytechnic University,National Engineering Research Center of Seafood,Dalian 116034,Liaoning)

机构地区：[1]大连工业大学食品学院、国家海洋食品工程技术研究中心,辽宁大连116034

出　　处：《中国食品学报》2023年第9期67-78,共12页Journal of Chinese Institute Of Food Science and Technology

基　　金：“十三五”国家重点研发计划重点专项(2017YFD0400100)。

摘　　要：以环磷酰胺(CTX)诱导免疫抑制BALB/c小鼠为模型,研究长白山野生松茸低温水提物(TMWE)及其粗蛋白组分(TMWE-CP)的体内免疫调节活性。结果表明,TMWE和TMWE-CP组分均可有效拮抗CTX的免疫抑制作用,使免疫抑制小鼠的体质量和免疫器官指数得到不同程度的恢复,改善小鼠脾脏损伤,恢复和增强BALB/c小鼠的碳颗粒清除能力、迟发型变态反应(DTH)、脾淋巴细胞增殖活性、血清半溶血值(HC50)以及血清IgG、IgM表达水平。TMWE和TMWE-CP组分可从非特异性免疫、细胞免疫及体液免疫3个方面起到增强免疫抑制小鼠免疫调节功能的作用。此外,在细胞免疫(DTH,脾淋巴细胞增殖活性)和体液免疫(HC50,血清IgG、IgM水平)方面,TMWE-CP组免疫调节效果显著优于TMWE组(P<0.05),而TMWE组能够更好地增强非特异性免疫(免疫器官指数和碳颗粒清除能力)。结论:TMWE和TMWE-CP组分可作为潜在的松茸源天然免疫调节剂,在针对性健康产品研发方面具有广阔的应用前景。The immunologic effects of Tricholoma matsutake water extract(TMWE)and Tricholoma matsutake water extract-crude protein(TMWE-CP)extracted from wild Tricholoma matsutake(T.matsutake)in Changbai Mountain with low temperature water,were investigated based on the immunosuppressed male BALB/C mice induced by cyclophosphamide(CTX).Compared with the immunosuppressed model control group,the experimental results showed that TMWE and TMWE-CP could slow down the decline of body weight,increased the splenic and thymic indices and alleviated the morpho-pathological characteristics of spleen exposed to CTX.TMWE and TMWE-CP could enhance and restore carbon particle scavenging ability,delayed type hypersensitivity,and splenic lymphocyte proliferation activity of BALB/C mice.The serum levels of IgG,IgM and serum half-hemolysis concentration of both groups were significantly increased(P<0.05).Therefore,they could enhance the immune ability of immunosuppressed mice from non-specific immunity,cellular immunity and humoral immunity.In addition,compared with TMWE group,TMWE-CP had more significant immunomodulatory effects in cellular immunity(delayed type hypersensitivity and splenic lymphocyte proliferation activity)and humoral immunity(serum half-hemolysis concentration and serum levels of IgG,IgM)(P<0.05),while TMWE group could better enhance non-specific immunity(immune organ index and carbon particle scavenging ability).In conclusion,TMWE and TMWE-CP might be used as potential natural immunomodulator of T.matsutake,and has wide application prospects in the utilization and development of effectivity-specific health products and immunomodulating agent of T.matsutake.

关 键 词：松茸低温水提物 BALB/c小鼠模型 非特异性免疫 细胞免疫 体液免疫 

分 类 号：R285.5[医药卫生—中药学]