离子色谱-脉冲积分安培法同时测定甘薯中10种可溶性糖组分  

Determination of Ten Kinds of Soluble Sugars in Sweetpotato by Ion Chromatography and Pulsed Amperometric Detector

作　　者：石彩玲 田侠[1] 孙一鸣 刘庆[1] Shi Cailing;Tian Xia;Sun Yiming;Liu Qing(College of Resources and Environmental Sciences,Qingdao Agricultural University,Qingdao 266109,Shandong)

机构地区：[1]青岛农业大学资源与环境学院,山东青岛266109

出　　处：《中国食品学报》2023年第9期324-330,共7页Journal of Chinese Institute Of Food Science and Technology

基　　金：现代农业产业技术体系专项资金项目(CARS-10-B11)。

摘　　要：为建立离子色谱-脉冲积分安培法(IC-PAD)同时检测提取液中10种可溶性糖组分(葡萄糖、果糖、半乳糖、甘露糖、鼠李糖、阿拉伯糖、蔗糖、麦芽糖、棉子糖和水苏糖)的方法,通过80%乙醇水溶液低温超声振荡对甘薯粉样品进行提取,以Thermo Dionex Carbo Pac PA20(3 mm×150 mm)阴离子交换色谱柱为分析柱,以Carbo Pac PA20(3 mm×50 mm)为保护柱,以不同浓度的NaOH、CH3COONa组合淋洗液进行梯度淋洗分离,柱温30℃,进样体积10μL,采用离子色谱-脉冲积分安培方法检测。结果表明:6种单糖线性范围0.02~10 mg/L,其它4种糖线性范围为0.02~20 mg/L,10种糖组分加标回收率94.5%~106.5%,相对标准偏差0.57%~1.89%。本方法线性范围广、操作简单、分析速度快、灵敏度高,重现性好,可用于对甘薯提取液中多种可溶性糖组分的同时测定。利用本方法对实际样品的测定结果表明,不同干燥方式对甘薯可溶性糖含量的影响不同,与冷冻干燥相比,热风干燥增加了甘薯样品中可溶性糖含量,还促进了其它糖分转化生成棉子糖和水苏糖两种功能性低聚糖。A method for the determination of soluble sugars in sweetpotato by ion chromatography and pulsed amperometric detector(IC-PAD)was established,and it can detect 10 kinds of soluble sugars(glucose,fructose,galactose,mannose,rhamnose,arabinose,sucrose,maltose,raffinose and stachyose)in sweetpotato at the same time.The sweetpotato powder samples were extracted by low temperature ultrasonic oscillation with 80%ethanol aqueous solution and membrane filtration.The analytical column was Carbo Pac PA20 Analytical Column(3 mm×150 mm),the column CarboPac PA20 Guard Column(3 mm×50 mm)was used as the protective column.The mobile phase was NaOH and CH3COONa,gradient elution procedure was used for separation and pulse integral amperometric for detection.The exchange column kept at 30℃,the injection volume 10μL,the eluates were detected with an Au working electrode.The linear range of six monosaccharides was 0.02-10 mg/L,the linear range of the other four sugars was 0.02-20 mg/L.The recoveries of these ten sugars from spiked samples was 94.5%-106.5%,relative standard deviation was 0.57%-1.89%.This method has the advantages of wide linear range,simple operation,fast analysis speed,high sensitivity and reproducibility.It can be used for the simultaneous determination of various soluble sugars in sweet potato extract.The determination results of actual samples by this method show that different drying methods have different effects on the soluble sugar content of sweet potato,compared with the freeze drying method,the hot air drying increases the soluble sugar content in sweetpotato and promotes the transformation of other sugars to produce raffinose and stachyose.

关 键 词：离子色谱 脉冲安培检测器 可溶性糖组成 食用型甘薯 

分 类 号：TS215[轻工技术与工程—粮食、油脂及植物蛋白工程] O657[轻工技术与工程—食品科学与工程]