Liraglutide Suppresses Myocardial Fibrosis Progression by Inhibiting the Smad Signaling Pathway  

作　　者：Wen SUN Hong MI De-ying HE Wen LI Yi-yan SONGYANG 

机构地区：[1]Department of Geriatrics,Chongqing Hospital of Traditional Chinese Medicine,Chongqing 400021,China [2]Department of Traditional Chinese Medicine,Chongqing Hospital of Traditional Chinese Medicine,Chongqing 400021,China [3]Department of Emergency,Renmin Hospital of Wuhan University,Wuhan 430060,China [4]Department of Pharmacy,Renmin Hospital of Wuhan University,Wuhan 430060,China

出　　处：《Current Medical Science》2023年第5期955-960,共6页当代医学科学（英文）

基　　金：supported by grants from the Natural Science Foundation of Hubei Province(No.2022CFB671);Health Research Project of Hubei Province(No.WJ2023F020);Hubei Province Key Laboratory Open Project(No.2021KFY023).

摘　　要：Objective Liraglutide is a commonly used hypoglycemic agent in clinical practice,and has been demonstrated to have protective effects against the development of cardiovascular disease.However,its potential role in myocardial fibrosis remains unexplored.The present study aims to assess the impact of liraglutide on the activation of cardiac fibroblasts.Methods Primary rat adult fibroblasts were isolated,cultured,and randomly allocated into 4 groups:control group,transforming growth factor beta1(TGFβ1)stimulation group,liraglutide group,and TGFβ1+liraglutide group.Fibroblast activation was induced by TGFβ1.Cell proliferation activity was assessed using the CKK-8 kit,and cellular activity was determined using the MTT kit.Reverse transcrition-quantitative polymerase chain reaction(RT-qPCR)was utilized to quantify the level of collagen transcription,immunofluorescence staining was performed to detect the expression level of type III collagen andα-smooth muscle protein(α-SMA),and immunoblotting was conducted to monitor alterations in signal pathways.Results The addition of 10,25,50 and 100 nmol/L of liraglutide did not induce any significant impact on the viability of fibroblasts(P>0.05).The rate of cellular proliferation was significantly higher in the TGFβl stimulation group than in the control group.However,the treatment with 50 and 100 nmol/L of liraglutide resulted in the reduction of TGFβl-induced cell proliferation(P<0.05).The RT-qPCR results revealed that the transcription levels of type I collagen,type III collagen,andα-SMA were significantly upregulated in the TGFβl stimulation group,when compared to the control group(P<0.05).However,the expression levels of these aforementioned factors significantly decreased in the TGFβl+liraglutide group(P<0.05).The immunofluorescence staining results revealed a significant increase in the expression levels of type III collagen andα-SMA in the TGFβl stimulation group,when compared to the control group(P<0.05).However,these expression levels significantly decreas

关 键 词：LIRAGLUTIDE myocardial fibrosis TGFβl SMAD 

分 类 号：R542.2[医药卫生—心血管疾病]