三叉神经痛琼脂压迫模型优化及相关解剖结构学研究  

Optimization of agar compression model for trigeminal neuralgia and related anatomical structural studies

作　　者：邓浩 罗星 石采灵 曾晨 杨汉丰[1] DENG Hao;LUO Xing;SHI Cailing;ZENG Chen;YANG Hanfeng(Radiology Department of Affiliated Hospital of North Sichuan Medical College,Nanchong 637000,China)

机构地区：[1]川北医学院附属医院放射科,南充637000

出　　处：《中国疼痛医学杂志》2023年第11期815-823,共9页Chinese Journal of Pain Medicine

基　　金：教育部产学合作协同育人项目(22097112074016);南充市市校合作项目(22SXQT0029)。

摘　　要：目的:通过3D定位建立琼脂压迫动物模型模拟三叉神经痛(trigeminal neuralgia,TN),利用影像学扫描进行模型验证,并通过采集不同模型的行为学、影像学及病理学等相关数据,对模型进行分析,以便于进一步探索TN的发病机制。方法:用von Frey测痛纤维丝筛选出痛阈值在8~15 g的雄性SD(Sprague Dawley,SD)大鼠32只,采用随机数字法分为实验组(16只)和对照组(16只),建立三叉神经根部琼脂压迫模型,术后1~7周通过von Frey测痛纤维丝测定大鼠机械疼痛阈值,术后4周、8周、12周进行灰质体积的基于体素形态学分析(voxel based management,VBM),采集术后8周和12周的三叉神经颅内段(intracranial segment,ICS)和眶下段神经(infraorbital nerve,ION)利用透射电镜(transmission electron microscope,TEM)进行超微结构分析。结果:机械疼痛阈值测定结果显示琼脂实验组术后机械刺激阈值出现降低,可持续至术后42天,对照组在术后14天内有轻度机械刺激阈值降低。TEM结果显示实验组于术后8周和12周三叉神经ICS和ION的电镜结果均观察到明显脱髓鞘改变,且术后12周较术后8周更严重。灰质体积VBM分析显示实验组大鼠术后4周脑灰质体积未出现明显变化;8周时左侧旁下托、左侧齿状回、左侧胼胝体压部、右侧顶叶联合皮质、下丘脑、右侧基底前脑、右侧纹状体及双侧初级躯体感觉皮质灰质体积显著变小;12周时上述变化范围明显扩大,甚至蔓延到双侧脑区;另外新观察到导水管周围灰质、左侧内嗅皮质、双侧扣带回及双侧海马的灰质体积显著变小;全脑分析显示各脑区无灰质体积增加。结论:琼脂压迫模型能产生稳定且较长持续时间疼痛,可作为继发性TN的可靠模型用于对TN发病机制、治疗等的进一步研究。ICS段神经损伤可经神经扩散或通过神经周围传导从而累及三叉神经ION段。短期内(8~12周)随时间延长,TN神经脱髓鞘损伤呈加重Objective:To further explore the pathogenesis of TN by establishing an agar compressed animal model through 3D localization to simulate trigeminal neuralgia(TN),validating the model with imaging scans,and analyzing the model by collecting behavioral,imaging,and pathological data from different models.Methods:Thirty-two male Sprague Dawley(SD)rats with pain thresholds ranging from 8 to 15 g were selected using von Frey pain filament measurement.They were randomly divided into the experimental group(16 rats)or control group(16 rats)to establish a trigeminal nerve root agar compression model.The mechanical pain threshold of the rats was measured using von Frey pain filaments at 1 to 7 weeks after surgery.Voxel based management(VBM)was performed on gray matter volume at 4,8,and 12 weeks after surgery.Intracranial segments(ICS)and infraorbital nerves(ION)of the trigeminal nerve were collected at 8 and 12 weeks postop-eratively for ultrastructural analysis with transmission electron microscopy(TEM).Results:Mechanical pain threshold results:the mechanical stimulation threshold of the agar experimental group decreased after surgery,lasting 42 days after surgery,while the agar control group showed a slight decrease in mechanical stimulation threshold within 14 days after surgery.TEM results:the experimental group had significantly demyelinating changes in the trigeminal nerve ICS and ION electron microscopy at 8 and 12 weeks after surgery,and at the 12 weeks after surgery more severe than that at 8 weeks after surgery.VBM analysis of gray matter volume:there was no significant change in the brain gray matter volume of the agar group rats at 4 weeks after surgery.At 8 weeks,the volume decreased significantly in the gray matter of the left lateral lower bracket,left dentate gyrus,left corpus callosum pressure,right parietal lobe combined cortex,hypothalamus,right basal forebrain,right striatum and bilateral primary somatosensory cortex.At 12 weeks,these changes significantly expanded and even spread to bilateral brain regio

关 键 词：三叉神经痛 动物模型 超微结构分析 

分 类 号：R745.11[医药卫生—神经病学与精神病学]