Structural and biomechanical characterizations of acellular porcine mitral valve scaffolds:anterior leaflets,posterior leaflets,and chordae tendineae  

作　　者：Bo Wang Leslie N.Sierad Jeremy J.Mercuri Agneta Simionescu Dan T.Simionescu Lakiesha N.Williams Ryan Vela Pietro Bajona Matthias Peltz Sharan Ramaswamy Yi Hong Jun Liao 

机构地区：[1]Department of Bioengineering,University of Texas at Arlington,Arlington,TX 76010,United States [2]Joint Department of Biomedical Engineering,Medical College of Wisconsin and Marquette University,Milwaukee,WI 53226,United States [3]Department of Bioengineering,Clemson University,Clemson,SC 29634,United States [4]Department of Biomedical Engineering,Florida International University,Miami,FL 33174,United States [5]Department of Biomedical Engineering,University of Florida,Gainesville,FL 32611,United States [6]Department of Cardiovascular and Thoracic Surgery,University of Texas Southwestern Medical Center,Dallas,TX 75390,United States [7]Allegheny Health Network-Drexel University College of Medicine,Pittsburgh,PA 15212,United States

出　　处：《Engineered Regeneration》2022年第4期374-386,共13页再生工程（英文）

基　　金：supported in part by NIH R15HL159599,R15HL140503,and AHA GRNT17150041,GRNT959644.

摘　　要：Mitral valve(MV)tissue engineering is still in its early stage,and one major challenge in MV tissue engineering is to identify appropriate scaffold materials.With the potential of acellular MV scaffolds being demonstrated recently,it is important to have a full understanding of the biomechanics of the native MV components and their acellular scaffolds.In this study,we have successfully characterized the structural and mechanical properties of porcine MV components,including anterior leaflet(AL),posterior leaflet(PL),strut chordae,and basal chordae,before and after decellularization.Quantitative DNA assay showed more than 90%reduction in DNA content,and Griffonia simplicifolia(GS)lectin immunohistochemistry confirmed the complete lack of porcine𝛼-Gal antigen in the acellular MV components.In the acellular AL and PL,the atrialis,spongiosa,and fibrosa trilayered structure,along with its ECM constitutes,i.e.,collagen fibers,elastin fibers,and portion of GAGs,were preserved.Never-theless,the ECM of both AL and PL experienced a certain degree of disruption,exhibiting a less dense,porous ECM morphology.The overall anatomical morphology of the strut and basal chordae were also maintained af-ter decellularization,with longitudinal morphology experiencing minimum disruption,but the cross-sectional morphology exhibiting evenly-distributed porous structure.In the acellular AL and PL,the nonlinear anisotropic biaxial mechanical behavior was overall preserved;however,uniaxial tensile tests showed that the removal of cellular content and the disruption of structural ECM did result in small decreases in maximum tensile modulus,tissue extensibility,failure stress,and failure strain for both MV leaflets and chordae.

关 键 词：Mitral valve tissue engineering Heart valve biomechanics Valve leaflets Chordae tendineae DECELLULARIZATION 

分 类 号：R318[医药卫生—生物医学工程]