生物强化提高水稻糙米锌含量及其生物有效性  

Effects of zinc biofortification fertilizer on zinc content and bioavailability of brown rice

作　　者：刘晓航 王张民 牛珊珊 宋佳平 张泽洲 刘志奎 庞敏 尹雪斌 LIU Xiao-hang;WANG Zhang-min;NIU Shan-shan;SONG Jia-ping;ZHANG Ze-zhou;LIU Zhi-kui;PANG Min;YIN Xue-bin(College of Agronomy,Shanxi Agricultural University,Taigu,Shanxi 030801,China;School of Environmental Science and Engineering,Suzhou University of Science and Technology,Suzhou,Jiangsu 215009,China;Jiangsu Bio-Engineering ResearchCenter for Selenium/Advanced Lab for Functional Agriculture,Suzhou Institute for Advanced Research,University of Science and Technology of China,Suzhou,Jiangsu 215123,China;Nanjing Institute for FAST/National Innovation Center for Functional Rice,Nanjing,Jiangsu 210031,China;School of Earth and Space Sciences,University of Science and Technology of China,Hefei,Anhui 230026,China;College of Resource and Environment,Anhui Science and Technology University,Chuzhou,Anhui 239000,China;Institute of Functional Agriculture(Food)Science and Technology(iFAST)at Yangtze River Delta,Anhui Science and Technology University,Chuzhou,Anhui 239000,China;Shanxi Functional Agriculture Research Institute,Shanxi Agricultural University,Taigu,Shanxi 030801,China)

机构地区：[1]山西农业大学农学院,山西太谷030801 [2]苏州科技大学环境科学与工程学院,江苏苏州215009 [3]江苏省硒生物工程技术研究中心/中国科学技术大学苏州高等研究院功能农业重点实验室,江苏苏州215123 [4]南京恒宝田功能农业产业研究院/国家粮食产业(功能稻米)技术创新中心,江苏南京210031 [5]中国科学技术大学地球和空间科学学院,安徽合肥230026 [6]安徽科技学院资源与环境学院,安徽滁州239000 [7]安徽科技学院长三角功能农业(食品)研究院,安徽滁州239000 [8]山西农业大学山西功能农业研究院,山西太谷030801

出　　处：《植物营养与肥料学报》2023年第10期1783-1793,共11页Journal of Plant Nutrition and Fertilizers

基　　金：国家农创园功能农业发展专项(NJGJNCY-FAST01);常熟市科技发展计划项目(CN202101);山西省农谷建设科研专项(SXNGJSKYZX201706);山西省重点研发计划重点项目(201703D211001-01-02)。

摘　　要：【目的】研究利用有机锌肥生物强化水稻糙米锌含量的可行性及其生物可给性。【方法】锌生物强化试验在江苏溧阳进行,供试锌肥为糖醇螯合态锌(Zn 170 g/L),供试水稻品种为中熟晚粳稻‘南粳46’。设5个处理:喷施清水(CK);锌肥喷施一次,用量为2.55 kg/hm^(2)(Zn1)、5.10 kg/hm^(2)(Zn2);锌肥喷施两次,总施用量为5.10 kg/hm^(2)(Zn3)、10.20 kg/hm^(2)(Zn4)。水稻成熟后,测定糙米中锌、植酸含量,测定糙米中锌赋存形态含量,计算糙米植酸/锌摩尔比、糙米锌赋存形态占比,并利用in vitro人工胃肠模拟法分析糙米锌在胃阶段和胃肠阶段的溶出量,以溶出锌与糙米锌量之比来计算糙米锌生物可给性。【结果】与CK处理相比,锌生物强化对糙米植酸含量无显著影响,但显著提高了糙米锌含量,Zn1、Zn2、Zn3和Zn4处理的增幅分别为23.93%、37.51%、82.38%和87.81%,Zn3和Zn4处理增幅差异不显著。Zn1、Zn2处理对糙米植酸/锌摩尔比无显著影响,Zn3和Zn4处理显著降低了植酸/锌摩尔比。锌处理不同程度提高了糙米中各赋存形态锌含量,Zn2处理显著提高了糙米盐溶态和碱溶态锌含量,Zn3处理显著提高了所有赋存形态锌含量,而Zn4处理显著提高了除水溶态锌外的其他3类锌含量,Zn3和Zn4处理间各赋存形态锌含量无显著差异。锌处理水平影响着糙米各赋存形态锌的占比,与CK处理相比,Zn1处理下各赋存形态锌占比无显著变化,Zn2处理显著降低了复合态锌占比,Zn3处理显著降低了水溶态锌占比,而Zn4处理显著降低了水溶态锌占比,显著提高了盐溶态锌占比。锌生物强化可提高糙米中锌在胃肠阶段的溶出量,CK、Zn1、Zn2、Zn3和Zn4处理强化后糙米锌在胃肠阶段的溶出量分别为19.52、24.15、23.14、30.62和32.55 mg/kg,Zn3和Zn4处理的溶出量无显著差异,但明显高于Zn1和Zn2处理。相关性分析表明,糙米锌在胃肠阶段溶出量与糙米锌含量极显著【Objectives】We studied the availability of zinc(Zn)biofortification of brown rice and the bioavailability of Zn in brown rice.【Methods】Zn biofortification experiment was carried out in Liyang County,Jiangsu Province.The tested Zn fertilizer was alditol chelated Zn(Zn 170 g/L),and the tested rice cultivar was mid-maturing late japonica rice‘Nanjing 46’.The treatments included one foliar application of Zn fertilizer at rate of 2.55(Zn1),and 5.10 kg/hm^(2)(Zn2),two foliar application at total rate of 5.10(Zn3)and 10.20 kg/hm^(2)(Zn4),and spraying water was used as control(CK).At maturing stage,brown rice samples were collected for measurement of the content of total Zn,four Zn fractions,and phytic acid(PA),and the molar ratio of phytic acid to Zn(PA/Zn)was calculated.The dissolution rate of brown rice Zn in the gastric and gastrointestinal stage was detected by in vitro gastrointestinal simulation method.【Results】Compared with CK,Zn biofortification did not significantly change the phytic acid content,but increased the total Zn content of brown rice.Zn1,Zn2,Zn3,and Zn4 treatment increased the total Zn content by 23.93%,37.51%,82.38%and 87.81%,respectively.Zn3 and Zn4 treatment had similar but significantly higher promotion effect than Zn1 and Zn2.Zn1 and Zn2 treatment did not change the PA/Zn ratio of brown rice significantly,while Zn3 and Zn4 treatment reduced the ratio.Zn treatments affected the contents of the four Zn fractions in brown rice to varying degrees.Zn2 increased the saltsoluble and alkali-soluble Zn content,Zn3 treatment increased all the four Zn fraction contents,and Zn4 treatment increased all the Zn fraction contents except water soluble Zn.Compared with CK,Zn1 had no significant impact on the proportion of Zn fractions;Zn2 reduced the proportion of complex Zn;Zn3 reduced the proportion of water-soluble Zn,Zn4 reduced the proportion of water-soluble Zn but increased that of salt-soluble Zn.Zn biofortification enhanced the dissolution amount of brown rice Zn at the gastrointestinal s

关 键 词：锌生物强化 糙米 锌生物有效性 植酸/锌摩尔比 锌赋存形态 胃肠锌溶出量 

分 类 号：S511[农业科学—作物学]