3种测试片对烟用香精香料菌落总数检测的适用性评价  被引量:1

Evaluating the applicability of three count plates for determining aerobic plate counts of tobacco flavors

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作  者:叶长文[1] 周芸[2] 李栋[1] 崔中月 赵婉霞 黄阔 胡中军[2] 周晓[2] YE Changwen;ZHOU Yun;LI Dong;CUI Zhongyue;ZHAO WanxiaHUANG Kuo;HU Zhongjun;ZHOU Xiao(Zhengzhou Tobacco Research Institute of CNTC,Zhengzhou 450001,China;Technology Center of China Tobacco Guangxi Industrial Co.,Ltd.,Nanning 530001,China)

机构地区:[1]中国烟草总公司郑州烟草研究院,郑州450001 [2]广西中烟工业有限责任公司技术中心,南宁市530001

出  处:《烟草科技》2023年第11期24-33,共10页Tobacco Science & Technology

基  金:广西中烟产学研科技项目“烟草添加剂卫生指示菌快速检测方法建立及应用研究”(GXZYCX2021C003)。

摘  要:为评价3M Petrifilm^(TM)菌落总数测试片、DNP菌落总数测试片和MC-Media菌落总数测试片对烟用香精香料菌落总数检测的适用性,分别对17株标准菌株、1个菌落总数定量质控样品和56个烟用香精香料样品的菌落总数进行检测,观察3种测试片和平板计数琼脂培养基上的菌落形态并计数,将菌落总数计算结果进行相关性分析和配对t检验,对菌落总数定量质控样品进行Z比分数评价,比较使用3种测试片和平板计数琼脂培养基检测样品中菌落总数的操作过程。结果显示:①除标准菌株中的枯草芽胞杆菌(Bacillus subtilis)、解淀粉芽胞杆菌(Bacillus amyloliquefaciens)和多黏类芽胞杆菌(Paenibacillus polymyxa)在3M Petrifilm^(TM)菌落总数测试片上的菌落有明显的液化现象外,其他标准菌株在3种测试片上呈现的菌落形态均较清晰,便于计数。相关性分析表明,标准菌株3种测试片的菌落总数对数值与平板计数琼脂培养基的菌落总数对数值呈极显著(P<0.01)正相关。②菌落总数定量质控样品的菌落总数对数值Z比分数评价结果均为“满意”。③烟用香精香料菌落总数的相关性分析和配对t检验表明,MC-Media菌落总数测试片与平板计数琼脂培养基的菌落总数对数值相关性(R^(2)=0.9228,P<0.01)最好,且差异不显著(P>0.05)。④3种测试片中,MC-Media菌落总数测试片使用最方便,检测耗时最短。综合比较3种测试片,MC-Media菌落总数测试片更适用于烟用香精香料菌落总数的快速检测。To evaluate the applicability of 3M Petrifilm^(TM),DNP and MC-Media count plates for the determination of aerobic plate counts of tobacco flavors,the aerobic plate counts of 17 standard bacterial strains,one quality control sample for aerobic plate count and 56 tobacco flavor samples were determined.The morphology of the colonies on the three count plates and plate count agar medium was observed and the total numbers of the colonies were counted separately.The results of aerobic plate count were analyzed by correlation analysis and paired t-test,and the results of quantitative quality control samples were evaluated based on Z-score.In addition,the procedures of using three count plates and plate count agar medium for determining the aerobic plate counts of the samples were compared.The results showed that:1)Except for the standard strains of Bacillus subtilis,Bacillus amyloliquefaciens and Paenibacillus polymyxa,which showed visible liquefaction on the 3M Petrifilm^(TM) count plate,most of the standard strains presented clear colony morphology on the three count plates and were easy to count.Correlation analysis showed that there was a significant(P<0.01)positive correlation between the logarithmic values of aerobic plate counts of the standard strains on the three count plates and the plate count agar medium.2)The results of the Z-score evaluation of the logarithmic values of the quality control samples for aerobic plate count were all“satisfactory”.3)Correlation analysis and paired t-test for aerobic plate counts of tobacco flavors showed that the logarithmic values of the aerobic plate counts of MC-Media count plate correlated best with those of the plate count agar medium(R^(2)=0.9228,P<0.01)and the differences were not significant(P>0.05).4)Of the three count plates,MC-Media count plate was the most convenient to use,lest time-consuming,and more suitable for the rapid determination of aerobic plate counts of tobacco flavors.

关 键 词:测试片 菌落总数检测 烟用香精香料 

分 类 号:TS264.3[轻工技术与工程—发酵工程]

 

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