芽孢杆菌ZYCHH-01发酵液提取物的抗菌抗氧化性能评价及应用  

Evaluation of antibacterial and antioxidant properties of Bacillus ZYCHH-01 fermentation extract and its application on facial cream

作　　者：高亚惠 吴夏泠 杨丹敏 刘群 翁武银[1] 张玉苍 GAO Yahui;WU Xialing;YANG Danmin;LIU Qun;WENG Wuyin;ZHANG Yucang(College of Ocean Food and Biological Engineering,Jimei University,Xiamen 363100,Fujian,China)

机构地区：[1]集美大学海洋食品与生物工程学院,福建厦门363100

出　　处：《精细化工》2023年第11期2445-2453,共9页Fine Chemicals

基　　金：国家自然科学基金项目(21978059);福建省科技计划高校产学研合作项目(2021N5013);科技部重点研发计划项目(2021YFD210020202/2021YFD210020404)。

摘　　要：芽孢杆菌及其细胞外代谢物具有抗氧化、抗菌等性能,已被用于护肤品中。通过盐酸沉淀法从芽孢杆菌ZYCHH-01发酵产物中提取出活性物质,以自由基清除率为指标评价提取物的体外抗氧化活性,通过牛津杯扩散实验评估提取物的抗菌能力,通过急性皮肤刺激和人体涂抹实验检测了添加提取物面霜的生物安全性。结果表明,该提取物对2,2-联苯基-1-苦基肼基自由基和2,2’-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐自由基具有一定的清除活性,其半抑制浓度(IC50)分别为0.732和0.267 g/L;对革兰氏阳性菌和阴性菌均有抑制作用,可使细菌形貌严重受损。在中国化妆品行业,单一的尼泊金酯最高质量分数为0.4%,混合酯的最高质量分数为0.8%,当提取物质量浓度为8g/L(质量分数约为0.8%)时,能有效抑制6种常见的致病菌生长,具有替代市售防腐剂的可行性;且所制备的面霜具有安全性、无皮肤刺激性。Bacillus and its extracellular metabolites have been used in skin care products due to their antioxidant and antibacterial properties.Herein,active substances from the fermentation product of Bacillus ZYCHH-01 were extracted by hydrochloric acid precipitation method,with their in vitro antioxidant activity evaluated using free radical scavenging rate as index,antibacterial performance assessed by Oxford cup diffusion test,and the biosafety of the extract-added cream examined by acute skin irritation and human application tests.The results showed that the extract exhibited certain scavenging activity against 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)free radicals with half maximal inhibitory concentration(IC50)of 0.732 and 0.267 g/L,respectively.Meanwhile,the extract inhibited the growth of both gram-positive and negative bacteria and severely destroyed the bacterial morphology.In the Chinese cosmetic industry,the maximum amount(mass fraction)of single nipagin ester and mixed ester used was 0.4%and 0.8%,respectively.The extract obtained with a mass concentration of 8 g/L(mass fraction about 0.8%)could effectively inhibit the growth of six common pathogenic bacteria,indicating their strong potential to replace commercially available preservatives.The facial cream added with the extract displayed biosafety and no skin irritation.

关 键 词：发酵提取物 芽孢杆菌ZYCHH-01 抗氧化性 抗菌性 面霜 生物工程 

分 类 号：TQ658.2[化学工程—精细化工]