高蛋白配合饲料替代饲喂对拟穴青蟹肠道菌群的影响  

Effect of a High Protein Content Compound Feed Substitution for Trash fish on Intestinal Microbiota in Mud Crab Scylla paramamosain

作　　者：苏贵森 李敏[1] 陈然[1] 胡高宇 肖国强[1,2] 蔡景波 张翔[1] SU Guisen;LI Min;CHEN Ran;HU Gaoyu;XIAO Guoqiang;CAI Jingbo;ZHANG Xiang(Zhejiang Key Lab of Exploitation and Preservation of Coastal Bio-Resource,Wenzhou Key Laboratory of Marine Genetics and Breeding,Zhejiang Mariculture Research Institute,Wenzhou 325005,China;National Demonstration Center for Experimental Fisheries Science Education,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]浙江省海洋水产养殖研究所,浙江省近岸水域生物资源开发与保护重点实验室,温州市海洋生物遗传育种重点实验室,浙江温州325005 [2]上海海洋大学水产科学国家级实验教学示范中心,上海201306

出　　处：《水产科学》2023年第6期955-965,共11页Fisheries Science

基　　金：浙江省重点研发计划项目(2019C02045;2018C02039).

摘　　要：为探明蟹公寓养殖模式下,高蛋白配合饲料替代杂鱼饲喂对拟穴青蟹菌群的影响,采用16S rRNA高通量测序技术,比较分析养殖过程中不同饵料组拟穴青蟹的肠道菌群结构。结果显示,各饵料组中优势菌群均为变形菌门、厚壁菌门、梭杆菌门和拟杆菌门,占操作分类单元总数85.84%以上。不同饵料组共有细菌操作分类单元(695个)占总数的18.92%,其序列数占总序列数的95.39%。主坐标分析显示不同饵料组肠道菌群的群落结构未产生显著分化。共现性网络分析显示,随养殖过程推进,微生物互作网络复杂度降低。功能预测分析显示,细菌次生代谢产物的生物合成、转运和分解代谢等功能主要在养殖前期出现显著性差异,而养殖后期饵料组间无显著性差异。试验结果表明,蟹公寓养殖过程中,不同饵料会对拟穴青蟹的肠道菌群造成影响。与杂鱼组相比,高蛋白配合饲料在维持肠道菌群多样性方面有一定优势,同时未显著影响肠道细菌群落结构和功能。To explore the differences of intestinal microbiota for Scylla paramamosain fed different diets(Eel feed-EF,and fresh fish-FF)under crab apartment,the microbial community structure and changes were studied by using the 16S rRNA gene amplicon sequencing technology in this study.The results showed that Proteobacteria,Firmicutes,Fusobacteria,and Bacteroidetes were the four most dominant categories at phylum level whose number of OTU accounted for more than 85.84%.The OTUs of common bacterial community accounted for 18.92%represented 95.39%of the sequences.The microbial community of different diets was no remarkably distinct by PCoA.With the pass of periods,the complexity of bacterial co-occurrence relationship decreased.The function analyses of intestinal microbiota showed that there was significant difference in secondary metabolites biosynthesis,transport and catabolism in early period,which was not significant in late period.Overall EF has no significant effect on the bacterial community structure and function.

关 键 词：蟹公寓 拟穴青蟹 饵料 肠道菌群 高通量测序 

分 类 号：S963.7[农业科学—水产养殖]