宫颈癌组织血管内皮生长因子受体-2、微小RNA-378表达及其预后评估价值  

The expression and prognostic value of vascular endothelial growth factor receptor-2 and microRNA-378 in cervical cancer patients

作　　者：孟丽 卢燕 王清清 Meng Li;Lu Yan;Wang Qingqing(Department of Pathology,Shaoxing Central Hospital,Shaoxing 312030,China)

机构地区：[1]绍兴市中心医院病理科,绍兴312030

出　　处：《中国医师进修杂志》2023年第10期925-930,共6页Chinese Journal of Postgraduates of Medicine

摘　　要：目的探讨宫颈癌组织血管内皮生长因子受体-2(VEGFR-2)、微小RNA-378(miR-378)表达及其预后评估价值。方法回顾性选择绍兴市中心医院2019年9月至2021年9月诊治的105例宫颈癌患者,分别取宫颈癌组织与癌旁正常组织(距肿瘤组织至少2 cm),采用免疫组织化学法检测组织中VEGFR-2表达,采用逆转录-聚合酶链反应(RT-PCR)法检测组织中miR-378表达。比较宫颈癌组织与癌旁正常组织VEGFR-2、miR-378表达水平,并分析VEGFR-2、miR-378表达与宫颈癌患者病理参数和预后的关系。以Kaplan-Meier法绘制生存曲线,采用单因素及多因素Cox回归分析影响宫颈癌患者预后的危险因素。结果宫颈癌组织VEGFR-2阳性表达率高于癌旁正常组织[86.67%(91/105)比62.86%(66/105)],miR-378表达水平高于癌旁正常组织(1.46±0.35比0.68±0.12),差异均有统计学意义(χ^(2)=15.77,t=21.60,P<0.05)。不同年龄、病理类型和肿瘤大小患者宫颈癌组织VEGFR-2、miR-378表达比较差异无统计学意义(P>0.05);随着临床分期增加、分化程度降低以及淋巴结转移的发生,VEGFR-2阳性表达率升高和miR-378表达水平升高(P<0.05)。以Kaplan-Meier法绘制生存曲线,结果显示miR-378高表达组总生存时间(OS)低于miR-378低表达组,VEGFR-2阳性表达组OS低于VEGFR-2阴性表达组,差异有统计学意义(P<0.05)。单因素及多因素Cox回归分析结果均表明临床分期Ⅲ~Ⅳ期、低分化、有淋巴结转移、VEGFR-2阳性表达和miR-378高表达是宫颈癌患者预后不良的独立危险因素(P<0.05)。结论VEGFR-2在宫颈癌组织中存在阳性表达,miR-378在宫颈癌组织中存在高表达,与临床分期、分化程度、淋巴结转移相关,同时VEGFR-2阳性表达、miR-378高表达会影响宫颈癌患者生存情况,是导致宫颈癌预后不良的危险因素。Objective To investigate the expression and prognostic value of vascular endothelial growth factor receptor-2(VEGFR-2)and microRNA-378(miR-378)in cervical cancer patients.Methods A total of 105 patients with cervical cancer diagnosed and treated in Shaoxing Central Hospital from September 2019 to September 2021 were selected.Cervical cancer tissues and adjacent normal tissues(at least 2 cm distant from the tumor tissue)were collected,respectively.Immunohistochemistry was used to detect the expression of VEGFR-2,and reverse transcription-polymerase chain reaction(RT-PCR)method was used to detect the expression of miR-378 in tissues.The positive expression rate of VEGFR-2 and the expression of miR-378 in cervical cancer tissues and adjacent normal tissues were compared,and the relationship of VEGFR-2 and miR-378 with pathological parameters and prognosis of cervical cancer was analyzed.Kaplan-Meier method was used to plot the survival curve,and univariate and multivariate Cox regression analysis was used to analyze the risk factors affecting the prognosis of patients with cervical cancer.Results The positive expression rate of VEGFR-2 in the cervical cancer tissues was higher than that in the adjacent normal tissues:86.67%(91/105)vs.62.86%(66/105);the expression level of miR-378 in the cervical cancer tissues was higher than that in the adjacent normal tissues:1.46±0.35 vs.0.68±0.12,there were statistical differences(χ^(2)=15.77,t=21.60,P<0.05).The positive expression rate of VEGFR-2 and the expression level of miR-378 in cervical cancer tissues had no relationship with age,pathological type and tumor size(P>0.05),but with the increase of clinical stage,the decrease of differentiation and the occurrence of lymph node metastasis,the positive expression rate of VEGFR-2 and the expression level of miR-378 increased(P<0.05).Survival curve was drawn by Kaplan-Meier method,the results showed that the overall survival time(OS)of patients with miR-378 high expression was significantly lower than patients with miR-378 lo

关 键 词：宫颈肿瘤 血管内皮生长因子受体-2 微小RNA-378 预后 

分 类 号：R737.33[医药卫生—肿瘤]