1,2-丙二醇对放射性口腔黏膜炎的防护作用研究  

Protective effect of 1,2-propanediol against radiation induced oral mucositis

作　　者：蒋瑛 闫福林 王训 邢爽 屠彦红[3] 宋若会[3] 申星 余祖胤 JIANG Ying;YAN Fulin;WANG Xun;XING Shuang;TU Yanhong;SONG Ruohui;SHEN Xing;YU Zuyin(Institute of Radiation Medicine,Academy of Military Medical Sciences,Academy of Military Sciences,Beijing 100850,China;Graduate School,Anhui University of Chinese Medicine,Hefei 230038,China;Department of Otorhinolaryngology,First Hospital Affiliated to Anhui University of Chinese Medicine,Hefei 230031,China)

机构地区：[1]军事科学院军事医学研究院辐射医学研究所,北京100850 [2]安徽中医药大学研究生院,合肥230038 [3]安徽中医药大学第一附属医院耳鼻咽喉科,合肥230031

出　　处：《军事医学》2023年第9期657-661,667,共6页Military Medical Sciences

基　　金：国家自然科学基金项目(81573088,81773366)。

摘　　要：目的研究1,2‐丙二醇对放射性口腔黏膜炎的防护作用。方法采用17 Gy X射线单次照射小鼠头颈部制备模型,观察1,2‐丙二醇6 g/kg腹腔注射预防给药(照前3 h)对小鼠放射性口腔黏膜炎的影响。将SPF级C57BL/6J小鼠随机分为对照组和给药组,每组8只,观察照后30 d小鼠体重和存活情况。病理取材观察实验分为正常组、照射对照组和给药组,每组4只,于照后7 d处死,取口腔黏膜进行甲苯胺蓝染色、HE染色、Ki67和P63免疫组化标记。结果17 Gy X射线照射后,对照组小鼠在11 d内全部死亡,1,2‐丙二醇预防给药可显著提高小鼠存活率,30 d存活率达到37.5%。形态学观察结果,正常组小鼠舌面未见溃疡,照射对照组小鼠照后7 d舌面溃疡成片,溃疡面积为(0.06±0.01)cm~2,而1,2‐丙二醇给药组舌面溃疡较少,溃疡面积为(0.01±0.01)cm~2。组织病理显示,照射对照组小鼠照后7 d舌黏膜上皮结构严重破坏,上皮厚度明显变薄,而1,2‐丙二醇给药组舌黏膜上皮结构保持完整,上皮厚度较照射对照组明显增加。Ki‐67及P63免疫组化结果显示,照射对照组小鼠照后7 d舌黏膜角化上皮增殖细胞和角化上皮干细胞数量极少,1,2‐丙二醇给药组小鼠舌黏膜角化上皮增殖细胞和角化上皮干细胞呈现明显增殖再生。结论1,2‐丙二醇对放射性口腔黏膜炎小鼠具有防护作用。Objective To assess the protective effect of 1,2‐propanediol against radiation‐induced oral mucositis in mice.Methods A murine model was established by subjecting the head and neck of mice to a dose of 17 Gy X‐rays,and the impact of intraperitoneal prophylaxis with 1,2‐propanediol at a dosage of 6 g/kg(administered 3 h prior to irradiation)on radiation‐induced oral mucositis in mice was assessed.Sixteen male C57BL/6J mice were randomly and evenly divided into the irradiation and drug administration group.Survival at 30 days and changes in the month after irradiation were recorded.Another 12 mice were divided into the normal group,irradiation group and drug administration group,with 4mice in each group.These mice were sacrificed 7 days after irradiation.The oral mucosa was stained with toluidine blue,HE staining,Ki67 and P63 immunohistochemistry.Results All the mice in the control group died within 11 days under17 Gy irradiation.1,2‐propanediol administration significantly improved the survival of mice,and the 30 d survival rate reached 37.5%.The results of morphological observation showed no ulcers on the tongue of mice in the control group,but revealed(0.06±0.01)cm~2ulcers on the tongue of mice in the irradiation group,and the ulcers were(0.01±0.01)cm~2on the tongue of mice in the 1,2‐propanediol group at day 7 after irradiation.The histopathological results showed that the structure of tongue mucosa epithelium in the irradiation group was severely damaged with thinner epithelial thickness,but was conserved with less thinned epithelial thickness in the 1,2‐propanediol group.Ki‐67 and P63 immunohistochemical results showed that keratinocyte proliferation cells and keratinocyte stem cells at day 7 after irradiation in the irradiation group were barely visible,while those in the 1,2‐propanediol group showed significant proliferation and regeneration.Conclusion 1,2‐propanediol has protective effect on radiation damage in mice with radiation‐induced oral mucositis.

关 键 词：放射性口腔黏膜炎 1 2-丙二醇 小鼠模型 

分 类 号：R781.5[医药卫生—口腔医学] R979.6[医药卫生—临床医学]