超声加速卢戈氏碘液增强Micro-CT成像用于口腔鳞状细胞癌术中成像的可行性研究  

Feasibility Study on Intraoperative Lugol's Iodine Enhanced Micro-CT Imaging Accelerated by Ultrasound for Specimen of Oral Squamous Cell Carcinoma

作　　者：牛燕红 夏成万 章茜[1] 马冠宇 王育新[1] 胡勤刚[1] 蒲玉梅[1] NIU Yanhong;XIA Chengwan;ZHANG Qian;MA Guanyu;WANG Yuxin;HU Qingang;PU Yumei(Department of Oral and Maxillofacial Surgery,Nanjing Stomatological Hospital,Affiliated Hospital of Medical School,Nanjing University,Nanjing 210008,China)

机构地区：[1]南京大学医学院附属口腔医院、南京市口腔医院口腔颌面外科,江苏南京210008

出　　处：《口腔医学研究》2023年第11期982-987,共6页Journal of Oral Science Research

基　　金：江苏省重点研发计划项目(社会发展)(编号:BE2021609);南京市口腔疾病临床医学研究中心(编号:2019060009)。

摘　　要：目的:将超声引入卢戈氏碘液(Lugol’s iodine)增强Micro-CT成像中,评价其加速口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)样本Micro-CT增强成像的可行性。方法:首先,超声(3 W)作用于Lugol’s iodine(1%、3%、5%)染色Cal-27细胞过程中,作用时间为5、10、15 min。平均光密度值(average optical density,AOD)评价Cal-27染色差异。其次,选择36只Balb/c裸鼠[8周龄,(25±2)g]断颈法处死后分离小鼠舌体组织,按照随机分组分为超声组及无超声组,再以1%、3%、5%Lugol’s iodine染色(n=6),在染色后0.5 h和1 h行Micro-CT成像和CT值定量分析。选取南京市口腔医院口腔颌面外科的4例病理诊断为颊黏膜鳞状细胞癌的OSCC男性患者,纳入8块肿瘤样本,随机分为超声组和无超声组。以3%Lugol’s iodine浸染组织块,Micro-CT成像时间为染色后0.5 h、1 h、2 h、4 h。CNR值定量分析肿瘤和正常组织间的成像清晰度。结果:细胞实验结果显示,超声作用Cal-27在5 min和10 min时,1%、3%Lugol’s iodine的AOD值高于对照组(P<0.05)。动物实验结果显示,超声作用下小鼠舌体组织的Micro-CT成像的CT值高于对照组(P<0.05)。临床研究结果显示,OSCC标本超声作用3%Lugol’s iodine染色2 h的Micro-CT成像可见肿瘤和正常组织边界,同时HE切片可见分界线。结论:超声辅助Lugol’s iodine可加速Cal-27的浸润,与此同时超声辅助Lugol’s iodine增强Micro-CT成像用于OSCC样本术中切缘评价具有一定的临床可行性。Objective:To evaluate the feasibility of Lugol's iodine enhanced micro-CT imaging accelerated by ultrasound for the oral squamous cell carcinoma(OSCC)samples.Methods:Firstly,ultrasound(3W)was applied to accelerate Lugol's iodine(1%,3%,and 5%)staining of Cal-27 cell line for 5,10,and 15 min,respectively.The average optical density(AOD)was used to evaluate the staining difference.Then,thirty-six BALB/C nude mice[8 weeks old,(25±2)g]were randomly divided into ultrasound group and non-ultrasound group,which were stained by 1%,3%,and 5%Lugol's iodine respectively.Micro-CT imaging and CT quantitative analysis were performed at 0.5 h and 1 h after staining.Finally,four male OSCC patients with buccal mucosa squamous-cell carcinoma were selected and randomly divided into ultrasound group and non-ultrasound group.Micro-CT images were obtained at 0.5 h,1 h,2 h,and 4 h after staining with 3%Lugol's iodine,and CNR was used to quantitative analyze the image clarity between tumor and normal tissue.Results:When Cal-27 was exposed to ultrasound for 5 min and 10 min,the AOD of 1%and 3%Lugol's iodine was higher than that of the control group(P<0.05).The value of micro-CT imaging of mouse tongue under ultrasound was higher than that of control group(P<0.05).The OSCC specimen results showed that the tumor and normal tissue boundaries were visible on micro-CT imaging with 3%Lugol's iodine staining for 2 h,while the dividing line was visible on HE sections.Conclusion:Ultrasound-assisted Lugol's iodine accelerates the infiltration of Cal-27,while ultrasound-assisted Lugol's iodine-enhanced micro-CT imaging provides some clinical guidance for intraoperative margin assessment of OSCC specimens.

关 键 词：超声 MICRO-CT 卢戈氏碘液 口腔鳞状细胞癌 切缘 

分 类 号：R739.8[医药卫生—肿瘤]