不同沙藏时间人参种子转录组分析及差异基因筛选  

Transcriptome analysis and differential gene screening of Panax ginseng seed in different sand storage stages

作　　者：尉广飞 李安宁 徐佳 陈士林[1] 董林林[1] WEI Guang-fei;LI An-ning;XU Jia;CHEN Shi-lin;DONG Lin-lin(Institute of Chinese Meteria Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Jilin Aodong Medicinal Seed Technology Co.,Ltd.,Yanbian 000623,China)

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]吉林敖东药材种业科技有限公司,吉林延边000623

出　　处：《药学学报》2023年第10期3130-3139,共10页Acta Pharmaceutica Sinica

基　　金：中央级公益性科研院所基本科研业务费专项资金资助项目(ZXKT21037,ZZ15-YQ-044,ZXKT22001)。

摘　　要：分析人参种子休眠解除过程中基因表达谱的变化情况,并筛选其差异基因,为解析人参种子休眠解除机制提供依据。以低温沙藏不同时间的人参种子为研究材料,利用转录组测序技术进行比较分析。转录组共获得80.97 Gb Raw reads和80.19 Gb Clean reads。主成分分析和相关性分析表明基因在不同发育时期具有不同的表达模式且存在显著差异。可视化集合图显示有46248个unigenes在四个时期共表达,有414、445、400和389个unigenes分别在低温沙藏0、8、14和28天特异表达。基于基因本体数据库功能注释显示差异表达基因主要参与不饱和脂肪酸生物合成进程、核质体和氧化还原酶活性等。基于京都基因与基因组百科全书数据库代谢途径显示差异表达基因主要参与过氧物酶体、蛋白激酶信号通路-植物、植物激素信号转导、核糖体、不饱和脂肪酸的生物合成、植物生理节律等代谢通路。人参种子休眠解除过程中需要不饱和脂肪酸生物合成和植物激素信号转导等多个生物过程的协同调控,构成复杂的休眠解除调控网络。不同沙藏时间人参种子转录组分析及差异基因的筛选,为解析人参种子休眠解除机制、开展人参分子育种奠定基础。Analyze the changes in gene expression profiles during the process of Panax ginseng seed dormancy release,and screen for differential genes,providing a basis for analyzing the mechanism of P.ginseng seed dormancy release.Comparative transcriptome analysis was conducted by using RNA-Seq sequencing technology in P.ginseng seeds stored at different low temperature.A total of 80.97 Gb of Raw reads and 80.19 Gb of Clean reads were obtained from the transcriptome.Principal component analysis and correlation analysis showed that there were significant differences in gene expression patterns at different developmental stages.Upset results showed that 46248 unigenes were co-expressed in four stages,and 414,445,400 and 389 unigenes were specifically expressed in O,8,14 and 28 days,respectively.Gene Ontology functional annotation showed that the differentially expressed genes were mainly involved in nsaturated fatty acid biosynthetic process,nuclear body and oxidoreductase activity.Encyclopedia of Genes and Genomes metabolic pathway showed that differentially expressed genes were mainly involved in peroxisome,mitogen-activated protein kinase signaling pathway-plant,plant hormone signal transduction,ribosome,biosynthesis of unsaturated fatty acid,circadian rhythm-plant and other metabolic pathways.In the process of P.ginseng seed dormancy release,multiple biological processes,such as unsaturated fatty acid biosynthesis and plant hormone signal transduction,are required to coordinate regulation,which constitutes a complex dormancy release regulation network.Transcriptome analysis and differential gene screening of P.ginseng seeds at different sand storage time laid a foundation for the analysis of P:ginseng seed dormancy release mechanism and molecular breeding.

关 键 词：人参种子 休眠解除 转录组 差异基因 功能注释 

分 类 号：R284[医药卫生—中药学]