miR-155-5p和TRIP13在结直肠癌组织中的表达及与预后的关系  

The relationships between the expression of miR-155-5p,TRIP13 and prognosis in colorectal cancer

作　　者：徐阳 李红云[1] 史铭 何波 XU Yang;LI Hongyun;SHI Ming;HE Bo(Department of Oncology,The Third Medical Center,PLA General Hospital,Beijing 100039,China)

机构地区：[1]解放军总医院第三医学中心肿瘤科,北京100039

出　　处：《西部医学》2023年第11期1673-1678,共6页Medical Journal of West China

摘　　要：目的探讨miR-155-5p及甲状腺激素受体因子13(TRIP13)在结直肠癌(CRC)中的表达水平及与预后的关系。方法选取2015年1月—2017年5月本院经病理确诊的78例CRC患者为研究对象。采用实时荧光定量PCR(qRT-PCR)法检测CRC组织及癌旁组织中的miR-155-5p、TRIP13 mRNA表达情况,免疫组化染色法测定CRC组织及癌旁组织中TRIP13蛋白表达情况;分析miR-155-5p、TRIP13蛋白表达对CRC患者预后的影响;采用Kaplan-meier分析组织中miR-155-5p、TRIP13蛋白对CRC患者预后的影响;采用Pearson检验分析CRC组织中miR-155-5p与TRIP13 mRNA表达水平的相关性;多因素Cox回归分析CRC患者预后的影响因素。结果CRC组织miR-155-5p、TRIP13 mRNA表达水平及TRIP13蛋白的阳性表达率明显高于癌旁组织(P<0.05);CRC组织中miR-155-5p、TRIP13蛋白表达与患者年龄、性别、肿瘤大小、肿瘤位置、浸润深度无关(P>0.05),与患者TNM分期、肿瘤分化、淋巴结转移具有显著相关性(P<0.05)。miR-155-5p高表达组5年内生存率(42.50%)低于低表达组(71.05%);TRIP13蛋白阳性组5年内生存率(43.48%)低于阴性组(75.00%);CRC组织中miR-155-5p与TRIP13 mRNA表达水平呈正相关(r=0.491,P<0.05);Cox回归显示,肿瘤分化程度、TNM分期、淋巴结转移、miR-155-5p、TRIP13是CRC患者预后的影响因素(P<0.05)。结论CRC组织中miR-155-5p、TRIP13表达上调,并与CRC的进展和患者的预后有关,检测miR-155-5p、TRIP13表达情况有助于患者的预后评估。Objective To investigate the expression levels of miR-155-5p and thyroid hormone receptor factor 13(TRIP13)in colorectal cancer and their relationship with prognosis.Methods A total of 78 patients with colorectal cancer who were pathologically diagnosed in our hospital from January 2015 to May 2017 were selected as the research objects.The expression of miR-155-5p and TRIP13 mRNA in colorectal cancer tissues and adjacent tissues was detected by real time fluorescent quantitative PCR(qRT-PCR).The expression of TRIP13 protein in colorectal cancer tissues and adjacent tissues was determined by immunohistochemical staining method;the influence of miR-155-5p and TRIP13 protein expression on the prognosis of patients with colorectal cancer was analyzed.Kaplan-meier analysis was used to analyze the effect of miR-155-5p and TRIP13 proteins on the prognosis of colorectal cancer patients.Pearson test was used to analyze the correlation between miR-155-5p and TRIP13 mRNA expression in colorectal cancer.Multivariate Cox regression analysis was used to analyze the prognostic factors of colorectal cancer patients.Results The expression levels of miR-155-5p,TRIP13 mRNA and the positive expression rate of TRIP13 protein in colorectal cancer tissues were significantly higher than those in adjacent tissues,and the differences were statistically significant(P<0.05).The expression of miR-155-5p and TRIP13 protein in colorectal cancer tissue was not correlated with the patient′s age,gender,tumor size,tumor location,and depth of invasion(P>0.05),and was significantly correlated with the patient′s TNM stage,tumor differentiation,and lymph node metastasis(P<0.05).The 5-year survival rate of miR-155-5p high expression group(42.50%)was lower than that of low expression group(71.05%).The 5-year survival rate of TRIP13 protein positive group(43.48%)was lower than that of negative group(75.00%).There was a positive correlation between miR-155-5p and TRIP13 mRNA expression in colorectal cancer(r=0.491,P<0.05).Cox regression showed that tu

关 键 词：miR-155-5p 甲状腺激素受体因子13 结直肠癌 免疫组织化学 

分 类 号：R735.3[医药卫生—肿瘤] R392.31[医药卫生—临床医学]