CRISPR-based diagnostics of different biomolecules from nucleic acids,proteins,and small molecules to exosomes  

作　　者：Yuanshou Zhu Meng Zhang Shujuan Guo Hong Xu Zhijun Jie Sheng-ce Tao 

机构地区：[1]Shanghai Center for Systems Biomedicine,Key Laboratory of Systems Biomedicine(Ministry of Education),Shanghai Jiao Tong University,Shanghai 200240,China [2]School of Biomedical Engineering,Med-X Research Institute,Shanghai Jiao Tong University,Shanghai 200030,China [3]Department of Pulmonary and Critical Care Medicine,Shanghai Fifth People’s Hospital,Fudan University,Shanghai 200240,China [4]Center of Community-Based Health Research,Fudan University,Shanghai 200240,China

出　　处：《Acta Biochimica et Biophysica Sinica》2023年第10期1539-1550,共12页生物化学与生物物理学报（英文版）

基　　金：supported by the grants from the National Key Research and Development Program of China(No.2020YFE0202200);the National Natural Science Foundation of China(Nos.31970130 and 82070024);the Natural Science Foundation of Shanghai(No.20ZR1443700);the Specialized Department Foundation of Minhang District(No.2020MWTZB02).

摘　　要：CRISPR-based detection technologies have been widely explored for molecular diagnostics.However,the challenge lies in converting the signal of different biomolecules,such as nucleic acids,proteins,small molecules,exosomes,and ions,into a CRISPR-based nucleic acid detection signal.Understanding the detection of different biomolecules using CRISPR technology can aid in the development of practical and promising detection approaches.Unfortunately,existing reviews rarely provide an overview of CRISPR-based molecular diagnostics from the perspective of different biomolecules.Herein,we first introduce the principles and characteristics of various CRISPR nucleases for molecular diagnostics.Then,we focus on summarizing and evaluating the latest advancements in CRISPR-based detection of different biomolecules.Through a comparison of different methods of amplification and signal readout,we discuss how general detection methods can be integrated with CRISPR.Finally,we conclude by identifying opportunities for the improvement of CRISPR in quantitative,amplification-free,multiplex,all-in-one,and point-of-care testing(POCT)purposes.

关 键 词：CRISPR/Cas molecular diagnostics biomolecule detection point-of-care testing 

分 类 号：R914[医药卫生—药物化学]