紫外线照射对羟基吡啶毒性效应的影响  

Study on mechanism of toxicity of pyridine compounds under ultraviolet irradiation

作　　者：苗东洋 沈方琳 陈晖[1,2] 陆松柳 李丹 MIAO Dongyang;SHEN Fanglin;CHEN Hui;LU Songliu;LI Dan(Department of Environmental Science and Engineering,Fudan University,Shanghai,200433,China;School of Environmental and Chemical Engineering,Shanghai University,Shanghai,200444,China;Shanghai Survey,Design and Research Institute Co.LTD,Shanghai,200335,China)

机构地区：[1]上海市大气颗粒物污染与防治重点实验室,复旦大学环境科学与工程系,上海200433 [2]上海大学环境与化学工程学院,上海200444 [3]上海勘测设计研究院有限公司,上海200335

出　　处：《环境化学》2023年第10期3265-3275,共11页Environmental Chemistry

基　　金：国家自然科学基金(22122601,92043301);上海市自然科学基金(20ZR1404300);上海市科委科技创新计划项目(21230712800)资助.

摘　　要：吡啶类化合物广泛存在于各种环境介质中,本文以羟基吡啶(2-羟基吡啶(2-py)、3-羟基吡啶(3-py)、4-羟基吡啶(4-py)和2,6-二甲基-3-羟基吡啶(2,6-dm-3-py))为研究对象,利用费氏弧菌和基因重组大肠杆菌,评估4种羟基吡啶经紫外线照射后急性毒性、遗传毒性和氧化应激效应变化规律.结果表明,2-py、3-py和2,6-dm-3-py经365 nm和312 nm紫外线照射后,毒性随照射时间延长而增强,其急性毒性相较于未照射前,分别增强了24.7%—99.4%和84.0%—99.9%(照射8 h后).而4-py经两种波长的紫外线照射后毒性变化不同,经365 nm紫外线照射后,急性毒性降低;但经312 nm紫外线照射8 h后,急性毒性增强77.7%,说明4-py经两种波长紫外线照射后生成了不同的降解产物.遗传毒性测试结果表明,4种羟基吡啶及其降解产物对DNA损伤和修复过程具有浓度依赖性,在20—100 mg·L^(−1)C浓度下,细胞SOS响应和氧化还原应激相关基因表达显著,但在6.4—10 mg·L^(−1)C浓度范围内,毒性相关基因表达变化不大,说明4种羟基吡啶及其降解产物在20—100 mg·L^(−1)C内可引起DNA损伤和氧化应激效应.Pyridine compounds are widely found in various environments.In order to understand the toxic effects in the environment and reveal the influence of ultraviolet(UV)light on their toxicity,the toxicities of 4 pyridine compounds(2-hydroxypyridine(2-py),3-hydroxypyridine(3-py),4-hydroxypyridine(4-py)and 2,6-dimethyl-3-hydroxypyridine(2,6-dm-3-py)and their degradation products after UV irradiation were evaluated by both Vibrio fischeri luminescence inhibition assay and genotoxicity assay using recombinant Escherichia coli,respectively.The results showed that the acute toxicities of 2-py,3-py and 2,6-dm-3-py increased by 24.7%—99.4%and 84.0%—99.9%after 365 nm and 312 nm UV irradiation for 8 h,respectively.The acute toxicity of 4-py was unchanged after irradiated by 365 nm UV for 8 h,on the contrary,increased by 77.7%after irradiation at 312 nm for 8 h.The DNA damage and repair process induced by the four pyridine compounds and their degradation products was concentration-depended.At the concentration of 20—100 mg·L^(−1)C,the SOS response and Redox stress-related genes were significantly expressed,but at the concentration of 6.4 mg·L^(−1)to 10 mg·L^(−1)C,the expression of toxicity-related genes showed little change,indicating that the four pyridine compounds and their degradation products at 20—100 mg·L^(−1)C could cause DNA damage and oxidative stress.

关 键 词：吡啶类化合物 费氏弧菌 重组大肠杆菌 毒性评估 紫外线照射 

分 类 号：X171.5[环境科学与工程—环境科学]