HBV相关肝细胞癌患者血清miR-504、miR-200a、IL-24水平及其与肝癌的相关性  

Analysis of serum levels and correlation of miR-504,miR-200a and IL-24 in patients with HBV-related hepatocellular carcinoma

作　　者：成晓梅 田红 李想 杨洁 CHENG Xiao-mei;TIAN Hong;LI Xiang;YANG Jie(Department of Gastroenterology,Air Force 986th Hospital,Xi′an 710054,China;Department of Nephrology,Air Force 986th Hospital,Xi′an 710054,China;Department of Obstetrics and Gynecology,Air Force 986th Hospital,Xi′an 710054,China)

机构地区：[1]空军军医大学空军第九八六医院消化内科,西安710054 [2]空军军医大学空军第九八六医院肾脏病科,西安710054 [3]空军军医大学空军第九八六医院妇产科,西安710054

出　　处：《肝脏》2023年第10期1186-1190,共5页Chinese Hepatology

摘　　要：目的探究乙型肝炎病毒(HBV)相关肝细胞癌患者血清微小RNA(miR)504、miR-200a、白细胞介素(IL)24水平及与肝癌的相关性。方法选取2020年2月至2023年2月于空军第986医院接受治疗且符合纳入、排除标准的HBV相关性肝细胞癌患者60例作为观察组,选取同期慢性HBV慢性感染者60例作为对照组。对比两组患者一般资料,实时荧光定量PCR测定患者血清miR-504及miR-200a水平,通过酶联免疫吸附实验测定患者血清IL-24水平。通过相关性分析及二元logistic回归分析筛选与HBV慢性感染者肝癌发生相关的危险因素,采用受试者工作特征曲线下面积(AUC)评价各因素对患者肝癌发生的诊断价值。结果观察组患者HBV DNA高于对照组[(21.87±4.64)×10^(3)比(13.61±3.36)×10^(3) IU/mL,P<0.05],余一般资料无显著差异。观察组患者血清miR-504(0.75±0.16比1.09±0.20)、miR-200a(0.79±0.24比1.01±0.28)及IL-24(187.65±19.04比213.25±20.27)水平均显著低于对照组患者。Spearman相关性分析及二元logistic回归分析表明血清miR-504、miR-200a及IL-24水平降低均是HBV慢性感染者肝癌发生的独立危险因素(P<0.05)。血清miR-504对HBV慢性感染者肝癌发生的诊断价值最高(AUC=0.898,P<0.05)。结论HBV相关肝细胞癌患者血清miR-504、miR-200a、IL-24水平显著降低,与HBV慢性感染者肝癌发生呈负相关,有一定诊断价值。Objective To investigate the serum levels of micro-RNA(miR)504,miR-200a and IL-24,and thier correlation in patients with hepatitis B virus(HBV)-related hepatocellular carcinoma.Methods We selected patients with HBV-related hepatocellular carcinoma who were treated in our hospital from February 2020 to February 2023 and met the inclusion and exclusion criteria as the observation group(N=60).The control group(N=60)consisted of patients with chronic HBV infection in the same period.The general data of the two groups were compared.The levels of serum miR-504 and miR-200a were measured by real-time fluorescence quantitative PCR,and the level of serum IL-24 was measured by enzyme-linked immunosorbent assay(Elisa).The risk factors associated with hepatocellular carcinoma in patients with chronic HBV infection were screened by correlation analysis and binary Logistic regression analysis.The diagnostic value of these factors was evaluated by the receiver working characteristic curve(ROC)and the area under the curve.Results The average HBV DNA load in the observation group was significantly higher than that in the control group(21.87±4.64 vs 13.61±3.36,P<0.05),but there was no significant difference in other general data.The serum levels of miR-504(0.75±0.16 vs 1.09±0.20),miR-200a(0.79±0.24 vs 1.01±0.28)and IL-24(187.65±19.04 vs 213.25±20.27)in the observation group were significantly lower than those in the control group.Correlation analysis and Logistic regression analysis showed that the decrease of serum miR-504,miR-200a and IL-24 levels were independent risk factors for hepatocellular carcinoma in patients with chronic HBV infection(P<0.05).ROC curve analysis showed that serum miR-504 had the highest diagnostic value for hepatocellular carcinoma in patients with chronic HBV infection(AUC=0.898,P<0.05).Conclusion The levels of miR-504,miR-200a and IL-24 in the serum of patients with HBV-related hepatocellular carcinoma showed a significant decrease.Moreover,each of these indicators exhibited a negative correlat

关 键 词：乙型肝炎病毒 微小RNA 白细胞介素24 肝癌 

分 类 号：R735.7[医药卫生—肿瘤] R512.62[医药卫生—临床医学]