国标法与试剂盒法检测脆肉鲩中羟脯氨酸含量的比较  

Experimental Comparison of Hydroxyproline Content in Crisped Grass Carp by National Standard Method and Kit Method

作　　者：赵丹霞 喻铭佳 梁祖培 刘辉 Zhao Danxia;Yu Mingjia;Liang Zupei;Liu Hui(Guangdong Testing Institute of Product Quality Supervision,Foshan 528300,China)

机构地区：[1]广东产品质量监督检验研究院,广东佛山528300

出　　处：《广东化工》2023年第22期151-153,156,共4页Guangdong Chemical Industry

摘　　要：比较国家标准GB/T 9695.23-2008《肉与肉制品羟脯氨酸含量测定》和试剂盒法定量检测脆肉鲩中羟脯氨酸含量的差异。采用试剂盒法和国标法测定脆肉鲩中羟脯氨酸含量,通过相对标准偏差(RSD)来比对两种方法的精密度,通过加标回收实验比对方法准确度。由精密度与准确度实验结果可知,国标法与试剂盒法的相对标准偏差分别为2.34%和7.75%,加标回收率分别为92%~109%和64%~81%。试验结果表明,相对于试剂盒法,国标法精密度和准确度更高,更适合用于检测脆肉鲩的羟脯氨酸含量。To compare the difference between GB/T 9695.23-2008 Determination of hydroxyproline content in meat and meat products and the kit method for quantitative detection of hydroxyproline(Hyp)in crisped grass carp.The content of Hyp in crisped grass carp was detected by kit method and national standard method.The precision of the 2 methods were compared by relative standard deviation(RSD),and the accuracy of the methods were compared by the recovery experiment.According to the results of precision and accuracy,the relative standard deviation of the national standard and kit were 2.34%and 7.75%,and the recovery rate range were 92%~109%and 64%~81%.The results showed that compared to the kit method,the national standard method had the higher precision and accuracy,which was more suitable for determination of Hyp content in crisped grass carp.

关 键 词：国标法 试剂盒法 脆肉鲩 羟脯氨酸 测定 

分 类 号：O65[理学—分析化学]