人参皂苷Rg1通过Wnt3a/β-catenin信号通路影响糖尿病大鼠肾损伤的机制研究  

Ginsenoside Rg1 Passes through Wnt3aβ-the Mechanism of the Effect of Catenin Signaling Pathway on Renal Injury in Diabetes Rats

作　　者：李雪倩[1] 禚映辰 高雷 杨妙妍 杨凡[1] LI Xue-qian;ZHUO Ying-chen;GAO Lei;YANG Miao-yan;YANG Fan(Department of Endocrinology,The Second Affiliated Hospital of Xi'an Jiaotong University,Xi'an,Shaanxi,710005,China;Department of Pharmacy,The First Affiliated Hospital of Xi'an Jiaotong University,Xi'an,Shaanxi,710061,China)

机构地区：[1]西安交通大学第二附属医院内分泌科,陕西西安710005 [2]西安交通大学第一附属医院药剂科,陕西西安710061

出　　处：《现代生物医学进展》2023年第17期3216-3220,共5页Progress in Modern Biomedicine

基　　金：陕西省自然科学基础研究项目(2022JM-589)。

摘　　要：目的:研究人参皂苷Rg1通过Wnt3aβ-catenin信号通路影响糖尿病大鼠肾损伤的机制。方法:将60只SD大鼠随机分为对照组、模型组、厄贝沙坦组、人参皂苷Rg1组。模型组、厄贝沙坦组、人参皂苷Rg1组经腹腔注射链脲佐菌素建立糖尿病模型,给予对照组、模型组生理盐水灌胃,厄贝沙坦组接受厄贝沙坦干预,人参皂苷Rg1组接受人参皂苷Rg1干预,各组均连续干预8周。比较各组大鼠血糖血脂水平、Wnt3a/β-catenin表达、肾损伤及肾脏炎症指标。结果:与对照组相比,人参皂苷Rg1组、厄贝沙坦组、模型组空腹血糖、总胆固醇、甘油三酯水平依次升高(P<0.05)。与对照组相比,人参皂苷Rg1组、厄贝沙坦组、模型组Wnt3a、β-catenin表达水平依次升高(P<0.05)。与对照组相比,人参皂苷Rg1组、厄贝沙坦组、模型组血肌酐、尿素氮、24 h尿蛋白水平依次升高(P<0.05)。与对照组相比,人参皂苷Rg1组、厄贝沙坦组、模型组C反应蛋白、单核细胞趋化蛋白-1、肿瘤坏死因子-α水平依次升高(P<0.05)。结论:人参皂苷Rg1可有效调节糖尿病大鼠血糖血脂水平,抑制肾脏炎症反应,缓解肾损伤,调控Wnt3a/β-catenin信号通路可能是其发挥作用的重要机制。Objective:Study on ginsenoside Rg1 through Wnt3aβ-The mechanism of catenin signaling pathway affecting renal injury in diabetes rats.Methods:60 SD rats were randomly divided into control group,model group,irbesartan group and ginsenoside Rg1 group for prospective study.The model group,irbesartan group and ginsenoside Rg1 group were intraperitoneally injected with streptozotocin to establish the diabetes model,and the control group and model group were given normal saline by gavage.The irbesartan group received the intervention of irbesartan,and the ginsenoside Rg1 group received the intervention of ginsenoside Rg1,all of which were continuously intervened for 8 weeks.Compare the blood glucose and lipid levels,Wnt3a of rats in each group/β-Catenin expression,renal injury,and renal inflammation indicators.Results:Compared with the control group,the levels of fasting blood glucose,total cholesterol,and triglycerides in the ginsenoside Rg1 group,irbesartan group,and model group increased sequentially(P<0.05).Compared with the control group,ginsenoside Rg1 group,irbesartan group,model group Wnt3aβ-The expression level of Catenin increased sequentially(P<0.05).Compared with the control group,the levels of serum creatinine,urea nitrogen,and 24-hour urine protein in the ginsenoside Rg1 group,irbesartan group,and model group increased sequentially(P<0.05).Compared with the control group,ginsenoside Rg1 group,irbesartan group,model group C-reactive protein,monocyte chemoattractant protein-1,tumor necrosis factor-αThe levels increased sequentially(P<0.05).Conclusion:Ginsenoside Rg1 can effectively regulate the level of blood glucose and lipid in diabetes rats,inhibit renal inflammatory reaction,alleviate renal injury,and regulate Wnt3aβ-Catenin signaling pathway may be an important mechanism for its function.

关 键 词：糖尿病 肾损伤 人参皂苷RG1 Wnt3a/β-catenin信号通路 

分 类 号：R-33[医药卫生] R692R587.2