茶树3个TPT基因的鉴定与表达  

Identification and expression analysis of 3 TPT genes in tea plant

作　　者：曹丹 刘艳丽[1] 马林龙[1] 李娟[2] 金孝芳[1] CAO Dan;LIU Yan-li;MA Lin-long;LI Juan;JIN Xiao-fang(Fruit and Tea Research Institute,Hubei Academy of Agricultural Sciences,Wuhan 430064,China;Key Laboratory of Tea Science of Ministry of Education/National Research Center of Engineering and Technology for Utilization of Botanical Functional Ingredients/Co-Innova-tion Center of Education Ministry for Utilization of Botanical Functional Ingredients/Key Laboratory for Evaluation and Utilization of Gene Re-sources of Horticultural Crops,Ministry of Agriculture and Rural Affairs of China/Hunan Agricultural University,Changsha 410128,China)

机构地区：[1]湖北省农业科学院果树茶叶研究所,武汉430064 [2]茶学教育部重点实验室/国家植物功能成分利用工程技术研究中心/植物功能成分利用省部共建协同创新中心/农业农村部园艺作物基因资源评价利用重点实验室/湖南农业大学,长沙410128

出　　处：《西南农业学报》2023年第9期1852-1858,共7页Southwest China Journal of Agricultural Sciences

基　　金：国家重点研发计划项目(2021YFD1601103);湖北省重点研发计划项目(2023BBB043,2021BBA241);湖北省农业科技创新中心项目(2021⁃620⁃000⁃001⁃024)。

摘　　要：【目的】进行茶树磷酸丙糖转运蛋白(Triose phosphate translocator,TPT)基因鉴定和表达模式分析,为探索其基因功能和茶树抗性育种提供理论参考。【方法】基于茶树全基因组序列,利用生物信息学方法筛选鉴定TPT基因,分析其在茶树不同组织中的表达特性及其在冷胁迫、干旱胁迫、盐胁迫、茉莉酸甲酯(MeJA)处理和硒处理中的表达情况。【结果】从茶树基因组中鉴定到3个含有TPT结构域的基因,序列长度为807~1635 bp,分别编码268~544个氨基酸;3个TPT蛋白均呈弱碱性或者碱性,均为稳定蛋白、疏水蛋白;亚细胞定位预测3个TPT蛋白位于质膜或叶绿体;系统进化分析将茶树TPT分为2组,基因结构和保守基序分析表明,3个茶树TPT基因结构和保守结构域大致相同;启动子顺式作用元件分析表明,茶树TPT基因的启动子区含有多个光反应、胁迫和激素等响应元件。基因表达分析发现CsTPT3在芽和叶片中有较高的表达,可能与茶树光合作用有关;CsTPT2在不同逆境下均呈现出显著下调表达的趋势,推测该基因在应对茶树低温、干旱、MeJA、盐和外源高浓度硒等胁迫过程中起着负调控作用。【结论】CsTPT基因可能参与了茶树响应逆境胁迫的过程。【Objective】The study aimed to identify triose phosphate translocator(TPT)genes and analyze their expression pattern,which can provide theoretical reference for exploring its function and resistance breeding of tea plant.【Method】Based on the tea plant genome se⁃quence,bioinformatics methods were used to screen and identify the TPT genes,and then,the expression characteristic in different tissues,and the response to cold stress,drought stress,salt stress,methyl jasmonate treatment and selenium treatment were analyzed.【Result】3 genes containing TPT domains were identified from the tea plant genome,with sequence lengths ranging from 807 to 1635 bp,encoding 268 to 544 amino acids,respectively.They were weakly basic or basic,and all were stable proteins and hydrophobins.Subcellular localization speculated that the 3 proteins were located in the plasma membrane or chloroplast.Besides,phylogenetic analysis divided CsTPT genes into two groups,and structure and conserved motif analysis indicated that the 3 TPT gene structures and conserved domains were roughly the simi⁃lar.Moreover,promoter cis⁃acting element analysis showed that the promoter region of CsTPT genes contained multiple light⁃responsive,stress⁃responsive and hormone⁃responsive elements.Gene expression analysis found that CsTPT3 was highly expressed in buds and leaves,which might be related to photosynthesis of tea plant.CsTPT2 was significantly down⁃regulated under different stress conditions,and it was specula⁃ted that the gene played a negative regulatory role in response to the stresses of low temperature,drought,MeJA,salt and high concentration exoge⁃nous selenium in tea plants.【Conclusion】The CsTPT genes might be involved in adverse stress of tea plant.

关 键 词：茶树 Triose phosphate translocator(TPT) 生物信息学 表达分析 功能 

分 类 号：S571.1[农业科学—茶叶生产加工]