高产聚苹果酸黑色素短梗霉CGMCC18996全基因组组装注释及关键蛋白分析  

Genome Assembly and Annotation of a High-Polymalic Acid(PMLA)Producing Strain Aureobasidium melanogenum CGMCC18996 and Analysis of Its Key Proteins Related to PMLA Synthesis

作　　者：王舸楠 李佳谦 李雨桐 陈世伟 王淑贤 赵廷彬 贾士儒[1,2,3] 乔长晟 WANG Genan;LI Jiaqian;LI Yutong;CHEN Shiwei;WANG Shuxian;ZHAO Tingbin;JIA Shiru;QIAO Changsheng(College of Bioengineering,Tianjin University of Science and Technology,Tianjin 300457,China;Key Laboratory of Industrial Fermentation Microbiology,Ministry of Education,Tianjin Key Laboratory of Industrial Microbiology,Tianjin 300457,China;Tianjin Engineering Center of Microbial Metabolism and Fermentation Process Control,Tianjin 300457,China;Tianjin Huizhi Baichuan Bioengineering Co.Ltd.,Tianjin 300457,China)

机构地区：[1]天津科技大学生物工程学院,天津300457 [2]工业发酵微生物教育部重点实验室暨天津市工业微生物重点实验室,天津300457 [3]天津市微生物代谢与发酵过程控制技术工程中心,天津300457 [4]天津慧智百川生物工程有限公司,天津300457

出　　处：《食品科学》2023年第16期213-219,共7页Food Science

基　　金：天津市科委基金项目(21YDTPJC00140);宁夏回族自治区重点研发计划项目(2022CMG02006,2021BEG03011);工业发酵微生物教育部重点实验室暨天津市工业微生物重点实验室开放课题(2020KF006)。

摘　　要：通过PacBio Sequel II及Illumina NovaSeq 6000测序平台对高产聚苹果酸(polymalic acid,PMLA)产黑色素短梗霉(Aureobasidium melanogenum)基因组进行测序,对测序得到的下机文件进行组装,并结合转录组数据进行基因结构注释。结果表明,产黑色素短梗霉基因组中共有6202个基因,主要参与碳水化合物转运及代谢、氨基酸转运代谢、转录后修饰、RNA加工及修饰等生物活动。功能注释结果显示基因组中有大部分基因与过氧化物体有关,进而对菌株进行透射电镜拍摄,发现菌体中存在圆形的类过氧化物体(乙醛酸体)结构,提示菌体可通过乙醛酸循环途径生成苹果酸。最后,对可能与PMLA合成有关的磷酸烯醇式丙酮酸羧化酶激酶和苹果酸合成酶进行了蛋白质结构预测,发现这两种蛋白可能具备合成苹果酸的能力。本研究结果可为产黑色短梗霉菌株的PMLA代谢提供一定的参考,同时,组装的基因组文件已上传至相关数据库,为后续的菌株开发利用提供基础。In this study,we applied PacBio Sequel II and Illumina NovaSeq 6000 sequencing platforms to sequence the genome of a high-polymalic acid(PMLA)-producing strain,Aureobasidium melanogenum CGMCC18996,and used different assemblers to obtain a high-quality genome assembly,which was then annotated using transcriptomic data.The results indicated a total of 6202 genes were found in the A.melanogenum genome,mainly involved in carbohydrate transport and metabolism,amino acid transport and metabolism,post-translational modification,RNA processing and modification.Meanwhile,functional annotation revealed that most genes in the genome were related to peroxisome in the strain.Transmission electron microscopy(TEM)indicated the existence of a circular peroxisome-like(glyoxysome)structure in the cells,demonstrating the ability to malic acid through the glyoxylate cycle.Finally,we predicted the protein structures of two enzymes related to PMLA biosynthesis,phosphoenolpyruvate carboxykinase(PCKA)and malate synthase(MASY).It was found that the enzymes could have the ability to synthesize malic acid.This study could provide a reference for metabolism regulation in A.melanogenum for improved PMLA production,and the assembled genome has been uploaded to the database,which could provide the basis for the future development and utilization of A.melanogenum CGMCC18996.

关 键 词：聚苹果酸 产黑色素短梗霉 基因组测序 基因组组装 蛋白质预测 

分 类 号：Q93[生物学—微生物学]