RGD多肽修饰钙磷陶瓷支架材料对骨质疏松兔成骨能力及骨功能影响的机制研究  

Study of Influences and Its Mechanism of RGD Peptide Modified Calcium Phosphate Ceramic Scaffold Material on Bone Forming Ability and Bone Function of Osteoporosis Rabbits

作　　者：侯睿 苗聪聪[2] 田振川[2] 赵雍 杨志海 HOU Rui;MIAO Cong-cong;TIAN Zhen-chuan;ZHAO Yong;YANG Zhi-hai(Department of Stomatology,Beijing Shijitan Hospital Affiliated to Capital Medical University,Beijing,100038,China;Animal Laboratories,Beijing Stomatological Hospital Affiliated to Capital Medical University,Beijing,100050,China)

机构地区：[1]首都医科大学附属北京世纪坛医院口腔科,北京100038 [2]首都医科大学附属北京口腔医院动物实验室,北京100050

出　　处：《现代生物医学进展》2023年第21期4007-4012,共6页Progress in Modern Biomedicine

基　　金：国家自然科学基金面上项目(81571814)。

摘　　要：目的:探究RGD多肽修饰钙磷陶瓷支架材料对骨质疏松(osteoporosis,OP)兔成骨能力和骨功能的影响极其可能的作用机制。方法:选取40只雌性SPF级新西兰大白兔,随机分为A组(正常对照组)和B组(OP组),每组各20只,再将A组随机分为A1组:sham组、A2组:sham+HA组、A3组:sham+BCP组、A4组:sham+RGD-BCP组,每组5只;B组随机分为B1组:OP组、B2组:OP+HA组、B3组:OP+BCP组和B4组:OP+RGD-BCP组,每组5只。B组采用去势法制备OP模型,检测相关指标。结果:A组兔术前和术后3个月时BMD无变化(P>0.05),B组兔术后3个月时的BMD值较术前下降(P<0.05)。自第4 d开始,A组和B组RGD-BCP支架材料组细胞的OD值高于HA和BCP支架材料(P<0.05),且B组之间的差异较A组更大(P<0.05)。Western Blot分析显示,A、B两组中RGD-BCP中整合素avβ3的表达高于HA和BCP(P<0.05),且B组之间的差异较A组更大(P<0.05)。B4和A4组p38 MAPK蛋白的相对表达均低于、Wnt5a和β-catenin表达均高于B2、B3和A2、A3组(P<0.05),此外,A4组p38MAPK蛋白的相对低于、Wnt5a和β-catenin表达高于B4组(P<0.05)。结论:RGD-BCP对兔BMSCs具有良好的相容性和吸附作用,对OP模型兔更显著;且RGD-BCP的机制可能与调控p38 MAPK/Wnt5a/β-catenin信号通路有关,为OP合并骨缺损的修复提供了新的思路和理论依据。Objective:To investigate the influences and its mechanism of RGD peptide modified calcium phosphate ceramic scaf-fold material on bone forming ability and bone function of osteoporosis(OP)rabbits.Methods:40 female new zealand rabbits were ran-domly divided into group A(normal control group)and group B(OP group),with 20 rabbits in each group.Group A were randomly divided into group A1(sham group),group A2(sham+HA group),group A3(sham+BCP group)and group A4(sham+RGD-BCP group);group B were randomly divided into group B1(OP group),group B2(OP+HA group),group B3(OP+BCP group)and group B4(OP+RGD-BCP group),with 5 rabbits in each group.In group B,the OP model was prepared by the castration method to detect the relevant indicators.Results:The BMD of group A preoperative and 3 months postoperative had no difference(P>0.05),the BMD of group B was decrease 3 months postoperative(P<0.05).The OD of RGD-BCP in group A and B were higher than HA and BCP from 4th day(P<0.05),and the dif-ference of group B was larger than group A(P<0.05).The expression of integrin avβ3 of RGD-BCP in group A and B were higher than HA and BCP(P<0.05),and the difference of group B was larger than group A(P<0.05).The relative expression of p38 MAPK of group B4 and A4 were lower,Wnt5a andβ-catenin were higher than group B2,B3 and A2,A3(P<0.05),besides,the relative expression of p38 MAPK of group A4 were lower,Wnt5a andβ-catenin were higher than group B4(P<0.05).Conclusion:RGD-BCP has good compatibility and adsorption on rabbit BMSCs,and is more significant for OP model rabbits;and the mechanism of RGD-BCP may be related to the regulation of p38 MAPK/Wnt 5 a/β-catenin signaling pathway,which provides a new idea and theoretical basis for the repair of OP with bone defect.

关 键 词：精氨酸-甘氨酸-天冬氨酸 骨质疏松 成骨能力 骨功能 

分 类 号：R-33[医药卫生] R68R318.08