ARTP诱变筛选高产透明质酸菌株  

High Throughput Screening of High-yield Hyaluronic Acid Producing Strain by Mutagenesis Using Atmospheric and Room Temperature Plasma(ARTP)

作　　者：李凡 周娜娜 张媛 王小艳 魏超 赵国淼 LI Fan;ZHOU Na-na;ZHANG Yuan;WANG Xiao-yan;WEI Chao;ZHAO Guo-miao(COFCO Nutrition&Health Research Institute,Beijing 102209,China;Beijing Key Laboratory of Nutrition,Health and Food Safety,Beijing 102209,China)

机构地区：[1]中粮营养健康研究院有限公司,北京102209 [2]营养健康与食品安全北京市重点实验室,北京102209

出　　处：《当代化工》2023年第10期2466-2470,共5页Contemporary Chemical Industry

基　　金：国家重点研发计划“合成生物学”重点专项,合成生物学自动化铸造平台关键技术研发(项目编号:2018YFA0902900)。

摘　　要：透明质酸(HA)是一种由N-乙酷葡萄糖胺(GIcNAc)和D-葡萄糖醛酸(GlcA)以β-1,3和β-1,4糖苷键交替连接组成的高聚合大分子黏性多糖,具有极强的保湿作用,被称为理想的天然保湿因子,是目前自然界中发现的化妆品用保湿性能最好的物质。马疫链球菌是目前透明质酸生产的主要菌种。菌株诱变是提升菌株性能或提高代谢产物的常用方法。采用ARTP对菌株进行诱变,通过改良CTAB比浊法进行菌株高通量初筛和摇瓶复筛,显著提高了透明质酸生产能力。经过3轮ARTP迭代诱变和筛选,最终使菌株HA产量提高了28.7%。Hyaluronan or hyaluronic acid(HA),is a glycosaminoglycan composed of repeating polymeric disaccharides of uronic sugar(D-glucuronic acid)and amino sugar(N-acetyl-D-glucosamine),both linked by glucoronidicβ-1,3 glycosidic bonds.Streptococcus equi subsp.zooepididicus is the main strain in HA production.Strain mutagenesis is a commonly used method to improve strain performance or enhance metabolic products.In this paper,ARTP was used to mutate the strain,and improved CTAB turbidimetric method was used for high flux initial screening and shake flask re-screening of the strain,significantly improving the production capacity of hyaluronic acid.After three rounds of ARTP iteration mutagenesis and screening,the HA production of the strain was ultimately increased by 28.7%.

关 键 词：透明质酸 ARTP 马疫链球菌 菌株诱变和筛选 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]