Genomic allele-specific base editing with imperfect gRNA  

作　　者：Xuxu Chen Dongdong Zhao Xueting Hou Ju Li Shiming Pu Jidong Fei Siwei Li Zuping Zhou Changhao Bi Xueli Zhang 

机构地区：[1]School of Life Sciences,Guangxi Normal University,Guilin,Guangxi 541006,China [2]Tianjin Institute of Industrial Biotechnology,Chinese Academy of Sciences,Tianjin 300308,China [3]National Technology Innovation Center of Synthetic Biology,Tianjin 300308,China [4]Key Laboratory of Systems Microbial Biotechnology,Chinese Academy of Sciences,Tianjin 300308,China [5]Hubei College of Chinese Medicine,JingZhou,Hubei 434020,China [6]College of Life Science,Tianjin Normal University,Tianjin 300387,China [7]Guangxi Universities Key Laboratory of Stem Cell and Biopharmaceutical Technology,Guangxi Normal University,Guilin,Guangxi 541006,China

出　　处：《Journal of Genetics and Genomics》2023年第10期799-802,共4页遗传学报（英文版）

基　　金：This work was financially supported by the National Key Research and Development Program of China(2018YFA0904900);the National Natural Science Foundation of China(32225031,32171449,81903776);the Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project(TSBICIP-KJGG-017);the Tianjin Natural Science Foundation(20JCYBJC00310);the Youth Innovation Promotion Association CAS(2022177).

摘　　要：CRISPR base editor(BE)techniques are a promising tool for precise cytosine(C)to thymine(T),adenine(A)to guanine(G),and C to G base editing(CBE,ABE,and GBE,respectively)without the use of a donor DNA template conversion(Komor et al.,2016;Nishida et al.,2016;Gaudelli et al.,2017;Kurt et al.,2021;Zhao et al.,2021).

关 键 词：BASE A GENOMIC 

分 类 号：Q943.2[生物学—植物学]