NK细胞水平及毒性与卵巢型子宫内膜异位症病变程度相关性  

The correlation of NK cell percentage and toxicity with the severity of ovarian endometriosis

作　　者：张丽姣 花茂方[2] 孟茜 王雷雷[1,2] 姚军 ZHANG Li-jiao;HUA Mao-fang;MENG Qian;WANG Lei-lei;YAO Jun(Department of Gynecology Yangzhou University Medical College,Yangzhou 225009,China;Department of Gynecology and Obstetrics Lianyungang Maternal And Child Health Hospital,Lianyungang 222062,China)

机构地区：[1]扬州大学医学院妇科教研室,扬州225009 [2]连云港市妇幼保健院妇产科,连云港222062

出　　处：《现代免疫学》2023年第5期413-416,共4页Current Immunology

基　　金：连云港市妇幼保健院青年英才培养基金资助项目(ky202005);南京医科大学康达学院2022年科研发展基金课题(KD2022KYJJZD27);上海市重点实验室开放课题基金资助项目(17DZ2273600)。

摘　　要：为探究分化群56(cluster of differentiation 56,CD56)、分化群107a(cluster of differentiation 107a,CD107a)和自然杀伤细胞2族成员D(natural killer group 2 member D,NKG2D)在卵巢型子宫内膜异位症(endometriosis,EM)患者在位内膜和异位内膜中的表达情况,探讨EM发生发展过程中NK细胞数量及毒性的变化和其中关联,收集连云港市妇幼保健院2018年1月至2020年12月40例卵巢型EM患者在位内膜、异位子宫内膜以及10例健康人子宫内膜组织,免疫组化法检测CD56、CD107a和NKG2D表达水平,分析其表达程度。结果显示,卵巢型EM患者CD56、CD107a和NKG2D的表达强度显著低于健康女性(均P<0.01),异位内膜组织CD56、CD107a和NKG2D表达强度低于其在位内膜(均P<0.01),且CD56、CD107a和NKG2D表达程度随着病变程度的增加逐渐下降(均P<0.01)。由此,卵巢型EM患者的异位内膜组织中存在CD56、CD107a和NKG2D的表达下调,并且与病变程度存在一定的关联,提示异位子宫内膜可能是通过NKG2D途径降低NK细胞的数量及毒性,进而诱导局部的免疫逃逸。This study aimed to explore the correlation of the expression of cluster of differentiation 56(CD56),CD107a,and natural killer group 2 member D(NKG2D)in eutopic and ectopic endometrium of patients with ovarian endometriosis(EM),and the changes and correlation of NK cell number and toxicity during the occurrence and development of EM.For this purpose,the eutopic and ectopic endometria of 40 ovarian EM patients and the endometria of 10 healthy women were collected from January 2018 to December 2020.The expression levels of CD56,CD107a,and NKG2D were detected by immunohistochemistry,and their expression degree was analyzed.The results showed that the expressions of CD56,CD107a,and NKG2D in patients with ovarian EM were significantly lower than those in normal control(all P<0.01).The expressions of CD56,CD107a,and NKG2D in ectopic endometrium were lower than those in eutopic endometrium(all P<0.01).Interestingly,the expressions of CD56,CD107a,and NKG2D gradually decreased with the increase of lesion degree(all P<0.01).Therefore,our results show that the expressions of CD56,CD107a,and NKG2D in ectopic endometrium of ovarian EM patients are down-regulated,and there is a significant correlation with the lesion degree,suggesting that ectopic endometrium may reduce the number and toxicity of NK cells through NKG2D pathway,which in turn induces local immune escape.

关 键 词：子宫内膜异位症 自然杀伤细胞 分化群56 分化群107a 自然杀伤细胞2族成员D 免疫组织化学 

分 类 号：R738.1[医药卫生—肿瘤]