延髓背侧网状核胶质细胞活化参与大鼠咬合干扰致口颌面痛觉敏感的中枢机制  

Activation of glial cells in dorsal reticular nucleus of the medulla oblongata involved in the central mechanism of oral and maxillofacial pain sensitivity induced by occlusal interference in rats

作　　者：许永伟[1] 范莹莹 曹烨[3] 陆支越[2] 金建秋[2] Xu Yong-Wei;Fan Ying-Ying;Cao Ye;Lu Zhi-Yue;Jin Jian-Qiu(Department of Stomatology,Peking University People's Hospital,Beijing 100044,China;Department of Stomatology,Beijing Hospital/National Center of Gerontology/Institute of Geriatric Medicine,Chinese Academy of Medical Sciences,Beijing 100730,China;Department of Prosthodontics,Peking University School and Hospital of Stomatology/Center for Oral and Jaw Functional Diagnosis/National Clinical Research Center for Oral Diseases/National Engineering Laboratory for Digital and Material Technology of Stomatology/Beijing Key Laboratory of Digital Stomatology,Beijing 100081,China)

机构地区：[1]北京大学人民医院口腔科,北京100044 [2]北京医院口腔科/国家老年医学中心/中国医学科学院老年医学研究院,北京100730 [3]北京大学口腔医学院口腔医院修复科/口颌功能诊疗研究中心/国家口腔疾病临床医学研究中心/口腔数字化医疗技术和材料国家工程实验室/口腔数字医学北京市重点实验室,北京100081

出　　处：《解放军医学杂志》2023年第10期1129-1134,共6页Medical Journal of Chinese People's Liberation Army

基　　金：中央高水平医院临床科研业务费(BJ-2022-177)。

摘　　要：目的探讨大鼠咬合干扰去除后痛觉敏感维持模型中延髓背侧网状核(DRt)胶质细胞活化参与口颌面痛觉敏感的中枢机制。方法24只SD雄性大鼠(180~200 g)随机分为假手术组(于1%戊巴比妥钠全麻下保持开口5 min,但不粘固牙冠)、痛觉敏感维持组[实验性咬合干扰(EOI)8 d后去除干扰]、痛觉敏感维持(EOI 8 d后去除干扰)+DRt损毁组,每组8只,行为学实验动物建模后于7、10、14 d测定各组大鼠自我赏罚行为学表现。另取9只SD大鼠分为假手术组、痛觉敏感维持组(施加EOI 8 d去除EOI前)、痛觉敏感维持组6 d(施加EOI 8 d并去除EOI后6 d),每组3只,左心室灌流后取材进行DRt脑区胶质纤维酸性蛋白(GFAP)和大鼠小胶质细胞特异性标志物(OX-42)的免疫荧光染色,并进行半定量分析星形胶质细胞和小胶质细胞的荧光强度和荧光面积。结果EOI 10 d时,与假手术组比较,痛觉敏感维持组总摄食时间明显缩短(P<0.05),痛觉敏感维持+DRt损毁组总摄食时间虽较假手术组缩短,但差异无统计学意义(P>0.05);EOI 14 d时,与假手术组比较,痛觉敏感维持组总摄食时间仍明显缩短(P<0.05),而痛觉敏感维持+DRt损毁组差异无统计学意义(P>0.05);与痛觉敏感维持组比较,痛觉敏感维持+DRt损毁组总摄食时间明显延长(P<0.05)。免疫荧光染色半定量分析显示,与假手术组比较,痛觉敏感维持组GFAP及OX-42的荧光面积、荧光强度差异均无统计学意义(P>0.05),而痛觉敏感维持组6 d的GFAP荧光面积、荧光强度及OX-42荧光面积均明显增高(P<0.05);与痛觉敏感维持组相比,痛觉敏感维持组6 d的GFAP及OX-42的荧光面积、荧光强度差异均无统计学意义(P>0.05)。结论DRt参与了大鼠EOI模型在去除咬合干扰后痛觉敏感的维持,其中DRt的星形胶质细胞和小胶质细胞活化是痛觉敏感维持的中枢机制。Objective To investigate the central mechanism of glial cells of the dorsal reticular nucleus(DRt)in the modulation of chronic orofacial pain after removal of experimental occlusal interference(EOI)in rats.Methods Twenty-four male SD rats(180-200 g)were randomly divided into three groups(8 in each group):sham group,EOI hyperalgesia maintaining group(occlusal interference appliance removed on day 8 after wearing 0.4 mm thick crowns),EOI hyperalgesia maintaining+DRt damage group(EOI hyperalgesia maintaining rats were injected with ibotenic acid to damage DRt).The non-reflexive behaviors of the three groups were evaluated by using orofacial operant test on 7,10,14 d after model establishment.Nine male SD rats were randomly divided into three groups(3 in each group):sham group,EOI hyperalgesia maintaining group(day 8 after wearing 0.4 mm thick crowns,before removal of EOI),EOI hyperalgesia maintaining group 6 d(6 days after EOI removed on day 8).DRt sections were obtained and processed for immunofluorescence staining for glai fibrillary acidic protein(GFAP)and OX-42.The levels of expression were hemi-quantitatively analyzed to evaluate the fluorescence area and fluorescence intensity of astrocytes and microglia.Results EOI hyperalgesia maintaining group and EOI hyperalgesia maintaining+DRt damage group exhibited lower feeding time in orofacial operant test,which implied hyperalgesia(P<0.05).The hyperalgesia in EOI hyperalgesia maintaining group persisted after the removal of EOI,and the difference was statistically significant at 10 d and 14 d compared with sham group(P<0.05),while the hyperalgesia in the EOI hyperalgesia maintaining+DRt damage group showed a rebound trend,and the difference was not statistically significant at 10 d and 14 d compared with sham group(P>0.05).The total feeding time at 14 d significant longer compared with the EOI hyperalgesia maintaining group(P<0.05).Semi-quantitative analysis of immunofluorescence staining showed that the fluorescence area and fluorescence intensity of GFAP and OX-42

关 键 词：咬合干扰 口颌面痛觉敏感 延髓背侧网状核 胶质细胞 

分 类 号：R78[医药卫生—口腔医学]