Intervention of Xuduan Zhongzi Formula on spermatogenesis epididymal morphological changes in a mice model of oligospermia  

作　　者：LIU Jin-yao HE Ming WANG Quan-sheng TANG Zhen-yu YANG De-fen 

机构地区：[1]Guangxi University of Chinese Medicine,Nanning 530200,China [2]Guilin Hospital of Traditional Chinese Medicine,Guilin 541002,China [3]The First Affiliated Hospital of Guangxi University of Chinese Medicine,Nanning 530023,China

出　　处：《Journal of Hainan Medical University》2023年第14期28-34,共7页海南医学院学报（英文版）

基　　金：Central Government Funds for Guiding Local Science and Technology Development(No.ZY20198022);Graduate Education Innovation Program of Guangxi University of Traditional Chinese Medicine(No.YCXJ2021067)。

摘　　要：Objective:To investigate the intervention of Xuduan Zhongzi Formula on the epididymis structure of model of oligospermia mice.Methods:Ten of the 45 male mice were labeled as the normal group,and the remaining 35 mice were injected with chloral hydrate for five consecutive days,and the randomly selected five mice were anesthetized with chloral hydrate.The left epididymis was removed,and a few sperm were found in the epididymis under the optical microscope,indicating successful construction of the model.They were randomly divided into three groups:the normal group,the L-carnitine group and the Xuduan group with 10 rats in each group.The the L-carnitine group and the Xuduan group were administrated with equivalent dose of human solvent,and the normal group and the model group were administrated with normal saline.After 8 weeks of intragastric administration,all experimental mice were killed,and the left epididymis was extracted for sperm detection,seminal plasma biochemical detection,HE staining,and electron microscopy.Results:In the model group,enlarged epididymal epithelial cells,vacuolar degeneration of primary and basal cells,and edema of interstitial cells and vascular dilation were found.Compared with the normal group,semen concentration,activity,SOD,A-glucosidase and fructose in the model group were significantly decreased,and the difference was statistically significant(P<0.01).After eight weeks of drug intragastric treatment,the semen concentration,activity,SOD,A-glucosidase and fructose in the Xuduan group were significantly increased compared with the model group,and the difference was statistically significant(P<0.01).Conclusion:Xuduan Zhongzi Formula can significantly improve the epididymal structure,the microenvironment of epididymal sperm maturation and the stability of epididymal epithelial structure of oligospermia model mice,creating conditions for sperm maturation in the epididymis.

关 键 词：Xuduan Zhongzi Formula OLIGOSPERMIA Ultrastructure of epididymis Sperm maturation 

分 类 号：R285.5[医药卫生—中药学]