脑性瘫痪儿童循环lncRNA NEAT1水平在语言认知康复训练疗效中的评估价值  

Value of circulating lncRNA NEAT1 in evaluating the language cognitive rehabilitation of cerebral palsy in children

作　　者：张敏[1] 唐亮[1] 戴燕琼 ZHANG Min;TANG Liang;DAI Yanqiong(Rehabilitation center,Children’s Hospital of Shanghai,Shanghai 200062,China)

机构地区：[1]上海市儿童医院,上海交通大学医学院附属儿童医院康复科,200062

出　　处：《河北医药》2023年第22期3423-3426,共4页Hebei Medical Journal

基　　金：上海市卫生计生系统需要薄弱学科建设(编号:2015ZB0402);上海交通大学转化医学交叉基金(编号:ZH2018QNA32)。

摘　　要：目的探讨lncRNA NEAT1在脑性瘫痪中的临床意义及其对语言认知康复训练疗效的评估价值。方法研究纳入63例脑性瘫痪儿童及50例健康儿童为对照组。通过实时定量PCR分析患儿及对照组儿童血清中lncRNA NEAT1的表达水平,运用ROC分析评估其对脑性瘫痪的诊断价值。对脑性瘫痪儿童施以语言认知康复训练,通过患儿训练前后定向能力、记忆力、注意力、语言功能、运动发育及智力发育变化评估语言认知康复训练的疗效。并通过χ^(2)检验评估lncRNA NEAT1对语言认知康复训练疗效的评估价值。结果脑性瘫痪患儿血清中lncRNA NEAT1的表达水平显著低于对照组儿童血清中的lncRNA NEAT1水平(P<0.001),且对脑性瘫痪具有显著的诊断价值(AUC=0.867)。语言认知康复训练显著提高了患儿的定向能力、记忆力、注意力、语言功能及运动与智力发育(P<0.05),且康复训练后患儿血清中lncRNA NEAT1表达水平显著升高(P<0.01)。lncRNA NEAT1升高的表达水平与患儿康复训练后提高的定向能力、记忆力、注意力、语言功能及运动与智力发育密切相关(P<0.05)。结论lncRNA NEAT1可作为脑性瘫痪的早期诊断生物标志物,且可有效评估语言认知康复训练疗效。Objective To investigate the clinical significance of the long noncoding RNA nuclear-enriched abundant transcript 1(lncRNAs NEAT1)in cerebral palsy in children and its value in evaluating language cognitive rehabilitation.Methods A total of 63 children with cerebral palsy and 50 healthy children(control)were included in this study.Serum lncRNA NEAT1 in children of both groups was measured by the real-time quantitative PCR.The diagnostic potential of lncRNA NEAT1 in cerebral palsy in children was assessed by plotting the receiver operating characteristic(ROC)curves.Children with cerebral palsy were given language cognitive rehabilitation.The therapeutic effect of the language cognitive rehabilitation was estimated by comparing the orientation,memory,attention,language function,motor and intellectual development before and after the rehabilitation.The value of lncRNA NEAT1 in evaluating the language cognitive rehabilitation of cerebral palsy in children was assessed by the Chi-square test.Results Serum lncRNA NEAT1 was significantly lower in children with cerebral palsy patients than that of control children(P<0.001),which possessed a significant diagnostic value in cerebral palsy(area under the curve[AUC]=0.867).The language cognitive rehabilitation significantly improved the orientation,memory,attention,language function,and motor and intellectual development in children with cerebral palsy(P<0.05).Serum lncRNA NEAT1 significantly increased after the language cognitive rehabilitation(P<0.01).The increase in serum lncRNA NEAT1 was significantly correlated with the improved orientation,memory,attention,language function,and motor and intellectual development in children with cerebral palsy(P<0.05).Conclusion LncRNA NEAT1 serves as an early diagnostic biomarker for cerebral palsy in children,which effectively evaluates the therapeutic effect of language cognitive rehabilitation.

关 键 词：脑性瘫痪 语言认知康复训练 lncRNA NEAT1 定向能力 记忆力 注意力 语言功能 运动发育 智力发育 

分 类 号：R742.3[医药卫生—神经病学与精神病学]